重组人促红细胞生成素鼻腔给药对大鼠慢性脑缺血的保护作用

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (9): 997-1001.

重组人促红细胞生成素鼻腔给药对大鼠慢性脑缺血的保护作用

叶夷露¹, 张琦¹, 季华¹, 张建亭¹, 黄春燕^1,2, 俞月萍¹, 饶艳¹

¹浙江医学高等专科学校,杭州 310053,浙江;
²温州医学院,温州 325035,浙江

收稿日期:2010-06-07 修回日期:2010-08-14 出版日期:2010-09-26 发布日期:2020-09-17
通讯作者: 张琦,女,硕士,副教授,硕导,研究方向:神经药理学。Tel: 0571-87692673, E-mail: zhangqiwzh@163.com
作者简介:叶夷露,女,硕士,讲师,研究方向:神经药理学。Tel: 0571-87692678, E-mail: yeyilu1006@126.com
基金资助:
浙江省卫生厅科研项目(2007A028、2008B034)

Protective effects of intranasal erythropoietin on chronic cerebral ischemia in rats

YE Yi-lu¹, ZHANG Qi¹, JI Hua¹, ZHANG Jian-ting¹, HUANG Chun-yan^1,2, YU Yue-ping¹, RAO Yan¹

¹Zhejiang Medical College, Hangzhou 310053, Zhejiang, China;
²Wenzhou Medical College,Wenzhou 325035, Zhejiang, China

Received:2010-06-07 Revised:2010-08-14 Online:2010-09-26 Published:2020-09-17

摘要/Abstract

摘要： 目的: 观察重组人促红细胞生成素(rhEPO)鼻腔给药对大鼠慢性缺血性脑损伤的保护作用。方法: 以大脑中动脉栓塞诱导大鼠局灶性脑缺血,手术当日术前30 min和术后2 h各给药一次,第2天起隔天一次,持续到术后14 d。缺血后1、3、7、14、21、28、35 d 进行神经症状评分和斜板试验,缺血后35 d计算脑梗死体积和缺血周边区神经元密度。结果: rhEPO鼻腔给药(24、48 U)、依达拉奉和rhEPO腹腔给药 (5000 U/kg) 能改善缺血后神经症状评分,提高斜板角度,降低脑梗死体积,增加缺血侧周边区存活神经元密度。结论: rhEPO鼻腔给药对大鼠慢性缺血性脑损伤具有保护作用,其有效剂量范围较腹腔给药低。

关键词: 重组人促红细胞生成素, 鼻腔给药, 脑缺血, 大鼠

Abstract: AIM: To investigate the protective effects of recombinant human erythropoietin (rhEPO) administrated intranasally on chronic cerebral ischemia in rats.METHODS: Focal cerebral ischemia in rats were induced by middle cerebral artery occlusion (MCAO). Drugs were injected intranasally or intraperitoneally 30 min before and 2 h after operation. From the second day, they were administrated qod for 2 weeks. In addition to neurological deficit and inclined board test during 35 d, we detected infarct volume and the survival neuron density in the ischemic brain tissue 35 d after operation.RESULTS: rhEPO (24, 48 U) intranasally, edaravone and rhEPO i.p. (5000 U/kg) significantly attenuated neurological deficit and infarct volume, increased the angle in the board test and the survival neuron density in the border of ischemia.CONCLUSION: rhEPO intranasally demonstrates protective effect on chronic cerebral ischemia in rats. Especially the effective dosage of rhEPO intranasally far is smaller than intraperitoneally.

Key words: Recombinant human erythropoietin (rhEPO), Intranasal administration, Cerebral ischemia, Rat

中图分类号:

R965.2

叶夷露, 张琦, 季华, 张建亭, 黄春燕, 俞月萍, 饶艳. 重组人促红细胞生成素鼻腔给药对大鼠慢性脑缺血的保护作用[J]. 中国临床药理学与治疗学, 2010, 15(9): 997-1001.

YE Yi-lu, ZHANG Qi, JI Hua, ZHANG Jian-ting, HUANG Chun-yan, YU Yue-ping, RAO Yan. Protective effects of intranasal erythropoietin on chronic cerebral ischemia in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(9): 997-1001.

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