金雀异黄素通过调控miR-27a及其靶基因对卵巢癌细胞SKOV3生长的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (12): 1321-1326.

• 基础研究 • 下一篇

金雀异黄素通过调控miR-27a及其靶基因对卵巢癌细胞SKOV3生长的影响

徐琳琳¹, 孙庆敏², 罗璇¹, 翟溯澜¹, 李萍¹, 赵小梅¹, 王雪融¹

¹南京医科大学基础医学院药理学系,南京 210029,江苏;
²南京医科大学附属无锡市妇幼保健院药剂科,无锡 214002,江苏

收稿日期:2012-09-16 修回日期:2012-11-09 发布日期:2012-12-31
通讯作者: 王雪融,通信作者,女,博士,教授,研究方向:肿瘤药理。Tel: 13813385723 E-mail: wangxrnj@gmail.com
作者简介:徐琳琳,女,硕士研究生,研究方向:肿瘤药理。Tel: 15996931516 E-mail: xulinlin16@163.com
基金资助:
国家自然科学基金项目(81001444; 30873099; 81102458; 81172004)

Effects of genistein on the growth of ovarian cancer cell SKOV3 by regulating miR-27a and target gene expression

XU Lin-lin¹, SUN Qing-min², LUO Xuan¹, ZHAI Su-lan¹, LI Ping¹, ZHAO Xiao-mei¹, WANG Xue-rong¹

¹Department of Pharmacology, Nanjing Medical University, Nanjing 210029, Jiangsu, China;
²Department of Pharmacy, Affiliated Wuxi Hospital for Maternal and Child Health Care of Nanjing Medical University, Wuxi 214002, Jiangsu, China

Received:2012-09-16 Revised:2012-11-09 Published:2012-12-31

摘要/Abstract

摘要： 目的: 研究金雀异黄素对卵巢癌细胞株SKOV3生长的抑制作用及其可能的机制。方法: 收集卵巢癌组织及良性组织,应用RT-PCR法检测miR-27a的表达。用不同浓度的金雀异黄素处理细胞,通过SRB法检测金雀异黄素单用或加入miR-27a mimics后对细胞株生长的抑制作用;经RT-PCR和Western blot检测miR-27a及靶基因Sprouty2表达的变化。结果: miR-27a在卵巢癌组织中的表达高于良性组织(P<0.05)。金雀异黄素可呈浓度和时间依赖的方式抑制卵巢癌细胞SKOV3生长;金雀异黄素浓度依赖性地下调miR-27a,上调Sprouty2的表达,且miR-27a mimics能够部分拮抗金雀异黄素抑制细胞生长的作用。结论: 金雀异黄素可能通过下调致癌miR-27a的表达,发挥抑制卵巢癌细胞生长的作用。

关键词: 金雀异黄素, 卵巢癌, 生长, miR-27a, Sprouty2

Abstract: AIM: To investigate the effects of genistein on the growth of ovarian cancer cell SKOV3 and potential mechanism. METHODS: The expression of the miR-27a in formalin-fixed paraffin-embedded (FFPE) ovarian cancer tissues and benign ovarian tissues were examined by RT-PCR. SKOV3 cells were treated with different concentration of genistein. Then the SRB assays were performed to assess the growth inhibition of genistein to ovarian cancer cells. RT-PCR and Western blot were used to test expression of miR-27a and Sprouty2 respectively. RESULTS: The expression levels of miR-27a in FFPE ovarian cancer tissues were significantly higher than those seen in benign ovarian tissues. Genistein could markedly decrease human ovarian cancer cell growth in time- and dose- dependent manner and downregulate the expression of miR-27a, which was accompanied by significantly increased expression of Sprouty2. CONCLUSION: Genistein has an inhibitory effect on the growth of ovarian cancer cells, which was due to the downregulation of miR-27a expression.

Key words: Genistein, Ovarian cancer, Growth, MiR-27a, Sprouty2

中图分类号:

R965.1

徐琳琳, 孙庆敏, 罗璇, 翟溯澜, 李萍, 赵小梅, 王雪融. 金雀异黄素通过调控miR-27a及其靶基因对卵巢癌细胞SKOV3生长的影响[J]. 中国临床药理学与治疗学, 2012, 17(12): 1321-1326.

XU Lin-lin, SUN Qing-min, LUO Xuan, ZHAI Su-lan, LI Ping, ZHAO Xiao-mei, WANG Xue-rong. Effects of genistein on the growth of ovarian cancer cell SKOV3 by regulating miR-27a and target gene expression[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(12): 1321-1326.

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金雀异黄素通过调控miR-27a及其靶基因对卵巢癌细胞SKOV3生长的影响

Effects of genistein on the growth of ovarian cancer cell SKOV3 by regulating miR-27a and target gene expression

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