γ-氨基丁酸信号在JAR细胞中的表达及对细胞侵袭能力的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (3): 274-279.

γ-氨基丁酸信号在JAR细胞中的表达及对细胞侵袭能力的影响

罗文萍, 刘真真, 彭洪英, 谭冬梅, 赵海, 谭毅

重庆医科大学实验动物中心,重庆 400016

收稿日期:2012-02-03 修回日期:2012-03-07 出版日期:2012-03-26 发布日期:2012-04-20
通讯作者: 谭毅,男,博士,研究员,主要从事胚胎植入与肿瘤侵袭转移的比较研究。Tel: 023-68486187, E-mail: tanyee66@hotmail.com
作者简介:罗文萍,女,博士研究生,主要从事胚胎植入机理的基础研究。E-mail: lwplucky@163.com
基金资助:
国家自然科学基金面上项目 (30270510;31171436);重庆市教委项目(KJ110316)

Expression and its significance of GABA signal in gestational trophoblastic cell pathological tissues

LUO Wen-ping, LIU Zhen-zhen, PENG Hong-ying, TAN Dong-mei, ZHAO Hai, TAN Yi

Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China

Received:2012-02-03 Revised:2012-03-07 Online:2012-03-26 Published:2012-04-20

摘要/Abstract

摘要： 目的: 本文通过研究γ-氨基丁酸(GABA)及其受体GABRP、GABAB1R在滋养层细胞株JAR中的表达,采用Transwell模型,给予GABA受体的激动剂和拮抗剂干预,探讨其对JAR细胞侵袭能力的影响。方法: 免疫细胞化学,RT-PCR,Western blot方法检测GABA、GABRP、GABAB1R滋养层细胞株JAR中的表达; Matrigel细胞侵袭模型检测GABA、GABA_AR,GABA_BR激动剂和拮抗剂对JAR细胞侵袭能力的影响。结果: GABA、GABRP、GABAB1R在细胞株JAR均有表达; GABA_A受体激动剂促进JAR细胞的侵袭,而GABA_B受体激动剂抑制JAR细胞的侵袭。结论: 在滋养层细胞株JAR中,GABA信号可能调节细胞的侵袭能力,从而参与滋养细胞相关妊娠疾病的发生发展。

关键词: JAR细胞, γ-氨基丁酸, 侵袭

Abstract: AIM: To explore the effects of GABA_R agonist or antagonist on JAR cells invasion by identifying the expression of GABA,π subunit of GABA_RA, B1 subunit of GABA_RB(GABAB1R) in JAR cells and taking Transwell chamber with GABA or receptor agonist or antagonists. METHODS: The protein expression of GABA, GABRP, GABAB1R were measured by immunocytochemistry and western bolt in JAR cells. The mRNA GABRP, GABAB1R was measured by RT-PCR. The effects of GABA and its receptors agonists and antagonists on JAR cells invasion were assay by Transwell chamber. RESULTS: GABA, GABRP, GABAB1R were expressed in JAR cells. GABA_A receptor agonist muscimol significantly promoted the invasion of JAR cells, while GABA_BR agonist baclofen significantly inhibited JAR cell migration. CONCLUSION: GABA signal may be associated with the pathogenesis of trophoblast-associated diseases of pregnancy.

Key words: Trophoblast diseases, γ-amino butyric acid, Invasion

中图分类号:

R965.2

罗文萍, 刘真真, 彭洪英, 谭冬梅, 赵海, 谭毅. γ-氨基丁酸信号在JAR细胞中的表达及对细胞侵袭能力的影响[J]. 中国临床药理学与治疗学, 2012, 17(3): 274-279.

LUO Wen-ping, LIU Zhen-zhen, PENG Hong-ying, TAN Dong-mei, ZHAO Hai, TAN Yi. Expression and its significance of GABA signal in gestational trophoblastic cell pathological tissues[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(3): 274-279.

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