当归挥发油对大鼠局灶性脑缺血再灌注损伤的保护作用

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (4): 387-391.

当归挥发油对大鼠局灶性脑缺血再灌注损伤的保护作用

罗慧英, 杨林, 杨焕, 刘渊

甘肃中医学院药理教研室,甘肃省中药药理与毒理重点实验室,兰州 730000,甘肃

收稿日期:2012-01-05 修回日期:2012-02-16 发布日期:2012-04-28
作者简介:罗慧英,女,博士,副教授,硕士生导师,主要从事保肝与抗肿瘤药物研究。Tel: 0931-8765395 E-mail: louria@126.com
基金资助:
兰州市科技局资助科研项目(2010-1-213)

Protection of volatile oil from Angelica sinensis on focal cerebral ischemia-reperfusion in rats

LUO Hui-ying, YANG Lin, YANG Huan, LIU Yuan

Department of Pharmacology, Gansu College of Traditional Chinese Medicine, Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicines of Gansu Province, Lanzhou 730000,Gansu,China

Received:2012-01-05 Revised:2012-02-16 Published:2012-04-28

摘要/Abstract

摘要： 目的: 探讨当归挥发油对大鼠局灶性脑缺血再灌注损伤的保护作用。方法: 采用改良线栓法阻断大鼠大脑中动脉建立脑缺血模型,到达再灌注时限后进行神经功能缺损评分;检测脑梗死体积比、脑含水量及脑血管通透性;血清NO、NOS含量;以及SOD和GSH-Px活性。结果: 当归挥发油可有效改善脑缺血再灌注损伤大鼠的神经功能缺损;降低脑梗死体积比、脑血管通透性和脑含水量;降低血清 NO、NOS含量; 增强SOD和GSH-Px活性。结论: 当归挥发油对脑缺血再灌注损伤有显著保护作用,其机制可能与提高脑组织抗氧化能力有关。

关键词: 当归挥发油, 脑缺血再灌注, NO, NOS, SOD, GSH-Px

Abstract: AIM: To discuss the protection of volatile oil from Angelica sinensis on focal cerebral ischemia-reperfusion in rats. METHODS: Focal cerebral ischemia-reperfusion injury model was made with suture-occluded method.The neurological deficits of rats were observed after occluding middle cerebral artery for 45 min and reperfusion for 2 hours.The volume of cerebral infarction were measured; the levels of NOS and NO, and the activities of SOD and GSH-Px in serum were detected. RESULTS: Volatile oil from Angelica sinensis could effectively ameliorate the changes induced by cerebral ischemia-reperfusion, including: meliorate the neurological deficits; decrease the levels of NOS and NO and the volume of cerebral infarction; increase the activities of SOD and GSH-Px. CONCLUSION: Volatile oil from Angelica sinensis shows the protection against ischemia-reperfusion injury, and the effect may related to increase antioxidation of the brain tissue.

Key words: Volatile oil from Angelica sinensis, Cerebral ischemia-reperfusion injury, NO, NOS, SOD, GSH-Px

中图分类号:

R965.2

罗慧英, 杨林, 杨焕, 刘渊. 当归挥发油对大鼠局灶性脑缺血再灌注损伤的保护作用[J]. 中国临床药理学与治疗学, 2012, 17(4): 387-391.

LUO Hui-ying, YANG Lin, YANG Huan, LIU Yuan. Protection of volatile oil from Angelica sinensis on focal cerebral ischemia-reperfusion in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(4): 387-391.

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