姜黄素对新生鼠坏死性小肠结肠炎模型的抗氧化作用及机制

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (1): 17-23.

姜黄素对新生鼠坏死性小肠结肠炎模型的抗氧化作用及机制

李金纯, 韦红, 王小玲, 郝静梅, 魏小娣

重庆医科大学附属儿童医院,儿童发育疾病研究省部共建教育部重点实验室,儿科学重庆市重点实验室,重庆市(儿童发育重大疾病诊治与预防)国际科技合作基地,新生儿疾病诊治中心,重庆 400014

收稿日期:2012-06-15 修回日期:2012-06-15 发布日期:2013-02-05
通讯作者: 韦红,通信作者,女,博士,副教授,硕士生导师,研究方向:新生儿疾病。 Tel: 023-63635576 E-mail: waehong@sina.com
作者简介:李金纯,女,硕士,主要从事新生儿疾病研究。 Tel: 15171420176 E-mail: babydouer@yahoo.cn
基金资助:
重庆市自然科学基金资助项目(2007BB5326)

The effect and possible mechanism of curcumin on experimental necrotizing enterocolitis

LI Jin-chun, WEI Hong, WANG Xiao-ling, HAO Jing-mei, WEI Xiao-di

Neonatal Diagnosis and Treatment Center, Children's Hospital of Chongqing Medical University; Ministry of Education Key Laboratory of Child Development and Disorders; Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002; Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China

Received:2012-06-15 Revised:2012-06-15 Published:2013-02-05

摘要/Abstract

摘要： 目的: 观察姜黄素作用于新生鼠坏死性小肠结肠炎(necrotizing enterocolitis,NEC)动物模型的疗效,初步探讨其可能的抗氧化作用机制。方法: 健康新生SD大鼠24只,随机分成3组,每组8只。Ⅰ组:NEC模型组;Ⅱ组:姜黄素干预组;Ⅲ组:正常对照组。采用三因素联合造模法。姜黄素干预组实验动物以腹腔注射进行干预。实验动物每日定时称量体重,观察并记录动物生长发育及一般情况。实验结束后进行肠组织标本大体观察及HE染色后组织损伤评分,测定肠组织中丙二醛(MDA)及超氧化物歧化酶(SOD)含量,免疫组化法检测肠道组织血红素加氧酶(HO-1)的表达。结果: Ⅱ组大鼠的生长发育、一般情况及肠组织大体形态改善均优于Ⅰ组。HE染色组织损伤评分显示Ⅰ、Ⅱ组均高于Ⅲ组,比较有统计学差异(P<0.05),Ⅰ、Ⅱ组比较差异有统计学意义(P<0.05)。MDA含量在Ⅰ、Ⅱ组较Ⅲ组明显升高,而Ⅱ组低于Ⅰ组(P<0.05)。SOD活性Ⅰ组较Ⅱ组和Ⅲ组显著下降(P<0.05),Ⅱ组与Ⅲ组比较差异无统计学意义。HO-1表达Ⅰ、Ⅱ组较Ⅲ组均明显上调(P<0.05),而Ⅱ组较Ⅰ组明显下降,差异具有统计学意义(P<0.05)。结论: 姜黄素是治疗NEC新生大鼠模型的有效药物。姜黄素可能通过诱导NEC实验动物肠黏膜HO-1的表达而发挥其对肠道组织的保护作用。

关键词: 坏死性小肠结肠炎, 姜黄素, 丙二醛, 超氧化物歧化酶, 血红素加氧酶

Abstract: AIM: To observe effects of curcumin on oxidative damage in intestines of newborn rats with necrotizing enterocolitis (NEC) , as well as investigate the potential mechanism of NEC. METHODS: Twenty four Sprague-Dawley (SD) rats were randomly divided into 3 groups right after birth. Rats in the groupⅠwere subjected to formula feeding, cold stress, hypoxic-reoxygenation treatment as well as LPS intragastric administration. NEC intestinal damage was evaluated by histological scoring. Animals in group II were managed according to the same way except the intraperitoneal injection with curcumin. Rats in Group Ⅲ were delivered naturally and were dam fed. All rats were weighed every day and sacrificed on the 4th day. Proximal intestines of ileocecal junction were undertaken pathological sectioning and evaluated for pathological scoring by hematoxylin and eosin (HE) staining. The activity of intestines malonaldehyde (MDA),superoxide dismutase (SOD) and the expression of heme oxygenase-1(HO-1) in all rats were also detected respectively. RESULTS: Compared with group Ⅲ, rats in groupⅡshowed better growing development, improved physical activity and milder intestine gross characteristics than groupⅠeven after the same stress. The pathological scores were significant different among these three groups (P<0.05). Intestinal MDA concentration increased with the severity of intestine injure (P<0.05). SOD activities of group I and II decreased than that of group III. There was a significant difference in the first two groups (P<0.05). The expression of HO-1 was significant increased in groupⅠand group Ⅱ.The difference between the two groups was statistical significant (P<0.05).CONCLUSION: Curcumin could alleviate the intestinal injure in the experimental necrotizing enterocolitis rats. The potentional mechanism might be reducing intestinal oxidative damage through upregulating the expression of HO-1, decreasing MDA concentration as well as enhancing the activity of SOD.

Key words: Necrotizing enterocolitis, Curcumin, Malonaldehyde, Superoxide dismutase, Heme oxygenase-1

中图分类号:

R965.2
R722

李金纯, 韦红, 王小玲, 郝静梅, 魏小娣. 姜黄素对新生鼠坏死性小肠结肠炎模型的抗氧化作用及机制[J]. 中国临床药理学与治疗学, 2013, 18(1): 17-23.

LI Jin-chun, WEI Hong, WANG Xiao-ling, HAO Jing-mei, WEI Xiao-di. The effect and possible mechanism of curcumin on experimental necrotizing enterocolitis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(1): 17-23.

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