银杏叶提取物对肝纤维化大鼠肝组织GRP78及CHOP表达的影响

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (4): 377-380.

银杏叶提取物对肝纤维化大鼠肝组织GRP78及CHOP表达的影响

叶蕾, 李剑霜, 陈芝芸, 严茂祥, 何蓓晖

浙江中医药大学第一临床医学院,杭州 310006,浙江

收稿日期:2013-08-07 修回日期:2014-04-07 出版日期:2014-04-26 发布日期:2020-07-24
作者简介:叶蕾,女,本科,副主任医师,研究方向:中西医结合肝纤维化。Tel:13757175668 E-mail:leihz88@163.com
基金资助:
浙江省中医药重点研究项目计划(2006Z0060);浙江省教育厅科研项目(Y201223352)

The role of ginkgo biloba extract on the hepatic GRP78 and CHOP expression in rats with liver fibrosis

YE Lei, LI Jian-shuang, CHEN Zhi-yun, YAN Mao-xiang, HE Pei-hui

First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, Zhejiang, China

Received:2013-08-07 Revised:2014-04-07 Online:2014-04-26 Published:2020-07-24

摘要/Abstract

摘要： 目的: 观察银杏叶提取物(GbE)对肝纤维大鼠内质网应激通路相关分子GRP78、CHOP表达的影响,探讨GbE抗肝纤维的作用机制。方法: 采用40％的CCl₄皮下注射(3 mL·kg^-1·d^-1,2次/周,首剂加倍)6周诱导大鼠肝纤维化模型,同时分别以200、100、50 mg·kg^-1·d^-1的GbE灌胃干预6周,空腹取肝组织,Real-time PCR及免疫组化法分别检测肝组织GRP78及CHOP基因的mRNA及蛋白表达。结果: 肝组织GRP78和CHOP的mRNA及蛋白的表达较正常组明显增加(P<0.05,P<0.01);应用高、中、低剂量GbE干预后,大鼠肝组织GRP78和CHOP基因的mRNA及蛋白表达显著下降(P<0.05,P<0.01)。结论: 肝纤维化大鼠肝脏发生了内质网应激反应,GbE下调肝纤维化大鼠肝组织内质网应激通路相关分子GRP78和CHOP的mRNA及蛋白的表达可能是其抗肝纤维化作用的机制之一。

关键词: 肝纤维化, 内质网应激, GRP78, CHOP, 银杏叶提取物

Abstract: AIM: To observe the effect of ginkgo biloba extract (GbE) on expression of GRP78 and CHOP in endoplasmic reticulum (ER) stress-related pathway in rats with liver fibrosis, and to explore the mechanism of anti-hepatic fibrosis of GbE. METHODS: The model with liver fibrosis in rats were induced by intraperitoneal injection of 40% CCl₄。Meanwhile, all the rats were administrated with different doses of GbE (200, 100 and 50 mg·kg^-1·d^-1 body weight) by gavage. After the experiment, the mRNA and protein expressions of hepatic GRP78 and CHOP were detected with RT-PCR and immunohistochemical method. RESULTS: The mRNA and protein expression of hepatic GRP78 and CHOP in the rats of the model were more obviously enhanced than in the normal (P<0.05, P<0.01), which were obviously decreased after the treatment with GbE (P<0.05, P<0.01). CONCLUSION: ERS reaction occurs on the liver of rats with liver fibrosis. And that GbE down-regulate the mRNA and protein expressions of hepatic GRP78 and CHOP probably is one of its mechanism of anti-liver fibrosis.

Key words: liver fibrosis, endoplasmic reticulum stress, GRP78, CHOP, GbE

中图分类号:

R965.2

叶蕾, 李剑霜, 陈芝芸, 严茂祥, 何蓓晖. 银杏叶提取物对肝纤维化大鼠肝组织GRP78及CHOP表达的影响[J]. 中国临床药理学与治疗学, 2014, 19(4): 377-380.

YE Lei, LI Jian-shuang, CHEN Zhi-yun, YAN Mao-xiang, HE Pei-hui. The role of ginkgo biloba extract on the hepatic GRP78 and CHOP expression in rats with liver fibrosis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(4): 377-380.

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