干扰survivin表达减少肿瘤细胞对紫外线诱导DNA损伤的修复作用

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (5): 497-502.

干扰survivin表达减少肿瘤细胞对紫外线诱导DNA损伤的修复作用

沈杰¹, 储冀汝², 贾元威², 刘晓平¹

¹皖南医学院药学院,芜湖 241001,安徽;
²皖南医学院弋矶山医院临床药学部,芜湖 241001,安徽

收稿日期:2014-01-11 修回日期:2014-05-12 出版日期:2014-05-26 发布日期:2014-06-05
通讯作者: 刘晓平,男,博士,教授,研究方向:肿瘤药学。Tel: 13855334156 E-mail: liuxiaoping@wnmc.edu.cn
作者简介:沈杰,男,博士,副研究员,研究方向:肿瘤药理学。Tel: 0553-5738350 E-mail: jie_shen23@aliyun.c
共同第一作者,储冀汝,女,硕士,研究实习员,研究方向:肿瘤药理学,分子生物学。Tel: 0553-5738350 E-mail: salmonella1985@163.com
基金资助:
国家自然科学基金项目(81272485),安徽省自然科学基金项目(1408085MH163),皖南医学院中青年自然科学基金项目(WK2012F09)

Interference of shRNA on survivin attenuate cellular DNA damage repair induced by ultraviolet radiation

SHEN Jie¹, CHU Ji-ru², JIA Yuan-wei², LIU Xiao-ping¹

¹Pharmacy School of Wannan Medical College, Wuhu 241001, Anhui, China;
²Department of Clinical Pharmacy, Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China

Received:2014-01-11 Revised:2014-05-12 Online:2014-05-26 Published:2014-06-05

摘要/Abstract

摘要： 目的:研究生存素(survivin)对紫外线(UV)诱导的肿瘤细胞DNA损伤修复作用及其机制。方法:本研究构建和包装survivin蛋白shRNA慢病毒载体;依据逐孔稀释法确定转染效率及滴度;以实时荧光定量法确定干扰效果;采用宿主细胞复活法(HCR)检测survivin蛋白表达对细胞整体DNA损伤修复能力的影响。结果:干扰survivin的表达显著降低肺腺癌H1299细胞对UV诱导DNA损伤的修复能力。结论:Survivin在UV诱导DNA损伤的修复作用中扮演重要角色。本研究可以为以survivin为靶点的肿瘤治疗提供一定的理论依据。

关键词: survivin, UV, 肿瘤细胞, DNA损伤修复

Abstract: AIM: To detect the DNA damage repair effect and its mechanism mediated by survivin during UV-induced apoptosis in tumor cells.METHODS: This study constructed and packaged lentiviral vector for survivin shRNA. Transfection efficiency and titer were measured by the dilution method. Real-time fluorescence quantitative method was applied to identify interference effects. Host-cells reactivating assay (HCR) was adopted to evaluate impact of survivin on cellular DNA damage repair capacity.RESULTS: Interference of survivin significantly reduced cellular DNA damage repair on lung adenocarcinoma H1299.CONCLUSION: Survivin plays an important role in DNA damage repair. Our research provides evidence for cancer therapy targeting survivin.

Key words: survivin, UV, tumor cell, DNA damage repair

中图分类号:

R965.2

沈杰, 储冀汝, 贾元威, 刘晓平. 干扰survivin表达减少肿瘤细胞对紫外线诱导DNA损伤的修复作用[J]. 中国临床药理学与治疗学, 2014, 19(5): 497-502.

SHEN Jie, CHU Ji-ru, JIA Yuan-wei, LIU Xiao-ping. Interference of shRNA on survivin attenuate cellular DNA damage repair induced by ultraviolet radiation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(5): 497-502.

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