外源性H2S供体NaHS对心肺复苏后大鼠学习记忆能力的影响

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (7): 727-732.

外源性H₂S供体NaHS对心肺复苏后大鼠学习记忆能力的影响

吴长江¹, 黄超发¹, 朱团结¹, 汪惠²

¹ 上海交通大学医学院苏州九龙医院重症医学科,苏州 215000,江苏;
² 第二军医大学附属长海医院麻醉科,上海200433

收稿日期:2013-08-07 修回日期:2014-06-20 发布日期:2014-07-21
通讯作者: 汪惠,女,硕士,主治医师,研究方向:重要脏器功能衰竭机制及其防治。 Tel: 18621995290 　E?mail: wanghuichanghai@gmail.com
作者简介:吴长江,男,本科,主治医师,研究方向:重要脏器功能衰竭机制及其防治。 Tel: 15862149539 E-mail: wcjicu@sina.com

Effects of exogenous H₂S donor NaHS on learning and memory in rats after cardiopulmonary resuscitation

WU Chang-jiang¹, HUANG Chao-fa¹, ZHU Tuan-jie¹, WANG Hui²

¹ Intensive Care Unit, Suzhou Kowloon Hospital Shanghai Jiaotong University Medical School, Suzhou 215000, Jiangsu,China;
² Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433,China

Received:2013-08-07 Revised:2014-06-20 Published:2014-07-21

摘要/Abstract

摘要： 目的研究外源性H₂S对心肺复苏(cardiopulmonary resuscitation,CPR)后大鼠记忆障碍的影响,并探究其作用机制。方法将SD大鼠随机分为5组,对照组、模型组(CPR)、低剂量NaHS治疗组(CPR+NaHS)、中间剂量NaHS治疗组、高剂量NaHS治疗组。采用Morris水迷宫法测定各组大鼠学习记忆能力;比色法检测大鼠大脑组织中超氧化物歧化酶(superoxide dismutase, SOD)活性及丙二醛(malonaldehyde, MDA)含量;HE染色观察海马组织形态学改变。结果与对照组比较,模型组大鼠在Morris水迷宫实验中,逃避潜伏期显著延长,在平台所在象限停留时间减少,穿越平台次数减少,SOD活性显著降低,MDA含量显著升高,海马神经元出现变性改变。与模型组比较,NaHS治疗组大鼠逃避潜伏期缩短,在平台所在象限搜索时间延长,穿越平台次数明显增多,SOD活性升高,MDA含量显著降低,海马神经元的变性得到改善。结论心肺复苏可损害大鼠的学习记忆能力,外源性H₂S供体NaHS减轻了CRP对大鼠学习记忆的损害,其作用机制可能与H₂S清除自由基和衰减脂质过氧化反应有关。

关键词: 心肺复苏, 硫化氢, Morris水迷宫, 学习记忆

Abstract: AIM: To investigate the effects of exogenous hydrogen sulfide on learning and memory in rats after cardiopulmonary resuscitation(CPR) and explore its mechanism. METHODS: Experimental rats were randomly divided into five groups: control group, model group(CPR), NaHS low dose group,middle dose group and high dose group . The ability of learning and memory was tested by Morris water maze; The activity of super oxide dismutase (SOD) and the content of malondialdehyde (MDA) were measured with colorimetry; the morphological alterations in hippocampus slices were assessed by microscope. RESULTS:Compared with control group, the escape latency in model group was longer, the residence time in original platform quadrant and the times of passing the position of platform were less, SOD activity was decreased and the content of MDA was increased in model group. Compared with model group, the escape latency in treatment groups was shorter, the number of crossings over the target area and the residence time in original platform quadrant were increased. SOD activity and the content of MDA was increased and decreased, respectively. Morphological changes were significantly attenuated by NaHS in treatment groups.CONCLUSION: CPR can impair the ability of learning and memory of rats, and exogenous H₂S donor NaHS can reduce the impairment of learning and memory of rats after CPR. The mechanism may be related to that H₂S has the effects of anti-lipid peroxidation and scavenging free radical.

Key words: cardiopulmonary resuscitation, hydrogen sulfide, morris water maze, learning and memory

中图分类号:

R965.2

吴长江, 黄超发, 朱团结, 汪惠. 外源性H₂S供体NaHS对心肺复苏后大鼠学习记忆能力的影响[J]. 中国临床药理学与治疗学, 2014, 19(7): 727-732.

WU Chang-jiang, HUANG Chao-fa, ZHU Tuan-jie, WANG Hui. Effects of exogenous H₂S donor NaHS on learning and memory in rats after cardiopulmonary resuscitation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(7): 727-732.

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外源性H₂S供体NaHS对心肺复苏后大鼠学习记忆能力的影响

Effects of exogenous H₂S donor NaHS on learning and memory in rats after cardiopulmonary resuscitation

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