EGFR基因rs1024750多态性显著影响钙加维生素D干预绝经后白人妇女的血钙水平

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (7): 772-779.

EGFR基因rs1024750多态性显著影响钙加维生素D干预绝经后白人妇女的血钙水平

王萍^{1, 2}, 徐潇静³, 卜锋啸³, 周宇^{3, 4}, 叶安^{3, 4}, 周伯庭^{1, 2, 3}

¹ 中南大学湘雅医院药学部,410008,湖南长沙;
² 中南大学药学院,410013,湖南长沙;
³ Osteoporosis Research Center, School of Medicine, Creighton University, 60¹ N 30th ST, Suite 4820, Omaha, NE 68131, USA;
⁴ Department of Biostatistics & Bioinformatics, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112,USA;

收稿日期:2013-12-12 修回日期:2014-07-02 发布日期:2014-07-21
作者简介:WANG Ping,female,postgraduate student,engaged in the study of clinical pharmacy and personalized medicine. Tel: 0371-84327265 E-mail:wangliu200798@126.com

SNP rs1024750 polymorphism in the EGFR gene significantly influences serum calcium levels in postmenopausal Caucasian women

WANG Ping^{1, 2}, XU Xiao-jing³, BU Feng-xiao³, ZHOU Yu^{3, 4}, YE An^{3, 4}, ZHOU Bo-ting^{1, 2, 3}

¹ Department of Pharmacy of Xiangya Hospital, Central South University, Changsha 410008, Hunan, China;
² School of Pharmacy, Central South University, Changsha 410013, Hunan, China;
³ Osteoporosis Research Center, School of Medicine, Creighton University, 60¹ N 30th ST, Suite 4820, Omaha, NE 68131, USA;
⁴ Department of Biostatistics & Bioinformatics, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112,USA;

Received:2013-12-12 Revised:2014-07-02 Published:2014-07-21
Contact: ZHOU Bo?ting,male,associate chief pharmacist,engaged in the study of clinical pharmacy and personalized medicine. Tel: 0371?84327265 　E?mail:botingzhou0918@126.com

摘要/Abstract

摘要： 目的考察EGFR基因多态性对钙加维生素D连续4年干预绝经后白人妇女血钙等人体成分的影响。方法本研究共招募美国中西部农村地区201名绝经后白人妇女,每日三次服用钙剂(乳酸钙 1 400 mg/d 或碳酸钙 1 500 mg/d)加维生素D(1 100 IU/d),170名受试者完成了4年的临床试验。用Haploview软件 4.2版挑选EGFR基因35个目标SNP并基因分型。每年对受试者的人体成分进行测试,分析目标SNP与人体成分的相关性。结果在人体成分中,仅有血钙基础水平与EGFR基因rs1024750位点基因多态性显著相关(P<0.05),血钙水平AA型>GA型>GG型。进行钙加维生素D干预后,血钙每年的变化值与该位点多态性显著相关,血钙变化值GG型>GA型>AA型。结论 EGFR基因rs1024750位点多态性与钙加维生素D干预绝经后白人妇女体内的血钙水平变化值显著相关。

关键词: EGFR, 基因, 单核苷酸多态性, 钙, 维生素D

Abstract: AIM: To study the effect of genetic polymorphisms of EGFR gene on the body composition, including blood calcium level, in postmenopausal Caucasian women through analyzing the data of a 4-year longitudinal supplementation of calcium and vitamin D. METHODS:A total of 201 postmenopausal non-Hispanic Caucasian women were recruited from rural Midwest area of the USA. They were administrated with calcium (either calcium citrate 1 400 mg/d or calcium carbonate 1 500 mg/d) and vitamin D (1 100 IU/d) supplementation three times per day for 4 years. One hundred-seventy subjects completed the 4-year clinical trial. A total of 35 candidate SNPs were selected with Haploview software 4.2 and genotyped. The cohort's body compositions after calcium and vitamin D intervention were measured every year. Association analysis between target SNPs and phenotypes of body composition was conducted.RESULTS: Among the cohort's body compositions, only serum calcium level at the baseline was significantly associated with rs1024750 SNP in EGFR gene (P<0.05), carriers of AA genotype had the highest baseline level while the GG genotype was the lowest. The annual changes of the serum calcium levels were significantly associated with rs1024750 SNP from Year 1 to Year 4 with calcium and vitamin D intervention (P<0.05), the serum calcium level of GG genotype had tremendous change, it was more than the total of AA and GA genotypes. CONCLUSION: The rs1024750 polymorphism in EGFR gene was significantly correlated with the changes of serum calcium level after calcium and vitamin D supplement in postmenopausal Caucasian women.

Key words: EGFR, genetics, single nucleotide polymorphism, calcium, vitamin D

中图分类号:

R969.1

王萍, 徐潇静, 卜锋啸, 周宇, 叶安, 周伯庭. EGFR基因rs1024750多态性显著影响钙加维生素D干预绝经后白人妇女的血钙水平[J]. 中国临床药理学与治疗学, 2014, 19(7): 772-779.

WANG Ping, XU Xiao-jing, BU Feng-xiao, ZHOU Yu, YE An, ZHOU Bo-ting. SNP rs1024750 polymorphism in the EGFR gene significantly influences serum calcium levels in postmenopausal Caucasian women[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(7): 772-779.

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