Shh、Gli1基因在多发性骨髓瘤中的表达及临床意义

中国临床药理学与治疗学 ›› 2015, Vol. 20 ›› Issue (3): 309-313.

Shh、Gli1基因在多发性骨髓瘤中的表达及临床意义

徐又海, 汪兴洪

皖南医学院弋矶山医院血液内科,芜湖 241001,安徽

收稿日期:2014-07-28 修回日期:2014-10-30 发布日期:2015-04-08
通讯作者: 汪兴洪,男,副教授,主任医师,研究方向:多发性骨髓瘤的发病机制。Tel: 13956159033 E-mail: yjswangxinghong@163.com
作者简介:徐又海,男,硕士,主治医师,研究方向:多发性骨髓瘤的发病机制。Tel: 13965190084 E-mail: dahai150987@sohu.com
基金资助:
皖南医学院中青年科研基金项目(WK2013F23)

Shh, Gli1 gene expression and clinical significance of multiple myeloma

XU You-hai, WANG Xing-hong

Department of Hematology, Yijishan Hospital, Wannan Medical College , Wuhu 241001, Anhui,China

Received:2014-07-28 Revised:2014-10-30 Published:2015-04-08

摘要/Abstract

摘要： 目的: 探讨Hedgehog-Gli信号通路Shh及Gli1基因在多发性骨髓瘤(MM)中的表达及意义。方法: 采用RT-PCR方法检测30例MM患者及20例非恶性血液病患者中Shh及Gli1 mRNA的表达,并分析二者与相关临床指标的关系。结果: Shh mRNA与Gli1 mRNA在初诊MM患者中高表达,与对照组比较差异具有统计学意义(P<0.01)。Shh mRNA与Gli1 mRNA表达量在DS分期及ISS分期中显示:Ⅲ期明显高于Ⅰ期或Ⅱ期(P<0.05);但A组和B组(P>0.05)中差异无统计学意义。Shh与乳酸脱氢酶(LDH)和β₂微球蛋白(β₂MG)呈正相关,与白蛋白和血红蛋白(Hb)呈负相关;Gil1与LDH、钙离子、CRP和β₂MG呈正相关,与白蛋白和Hb呈负相关;且Shh和 Gil1间存在正相关。结论: Shh及Gli1与MM的发生、发展有一定的关系,可能是MM靶向治疗的新的关注点。

关键词: Hedgehog-Gli信号通路, 多发性骨髓瘤, Shh, Gli1

Abstract: AIM: To investigate the expression of Shh and Gli1 gene of Hedgehog-Gli signaling pathway on multiple myeloma and its significance.METHODS: Detecting the Shh and Gli1 mRNA expression in 30 multiple myeloma patients and 20 non hematologic-malignancies patients by RT-PCR, and investigating relationships between Shh or Gli1 gene and clinical targets.RESULTS: Compared with the control group,the Shh and Gli1 mRNA expression in multiple myeloma patients were decreased significantly(P<0.01),which were higher in phase Ⅲ than in phaseⅠor Ⅱ (P<0.05) when staging by D-S and ISS.There is no obvious difference between group A and group B.There is positive correlation between Shh and LDH or β₂MG,but negative correlation between Shh and albumin or Hb.There is positive correlation between Gil1 and LDH, calcium ion,CRP or β₂MG, but negative correlation between Gil1 and albumin or Hb. As well,There is positive correlation between Shh and Gil1.CONCLUSION: Shh and Gil1 play a role in the development of multiple myeloma, which were maybe new locus in targeted therapy.

Key words: Hedgehog-Gli signaling pathway, multiple myeloma, Shh, Gli1

中图分类号:

R733.3

徐又海, 汪兴洪. Shh、Gli1基因在多发性骨髓瘤中的表达及临床意义[J]. 中国临床药理学与治疗学, 2015, 20(3): 309-313.

XU You-hai, WANG Xing-hong. Shh, Gli1 gene expression and clinical significance of multiple myeloma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(3): 309-313.

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