百事乐胶囊对抑郁模型大鼠海马隔颞轴BDNF、NT-3、NGF表达的影响

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (4): 418-423.

百事乐胶囊对抑郁模型大鼠海马隔颞轴BDNF、NT-3、NGF表达的影响

孟盼^1,2，柳卓^1,2，朱青^1,2，赵洪庆^1,2，张秀丽^1,2，雷昌^1,2，王宇红^1,2，杨蕙³

¹湖南中医药大学，长沙 410208, 湖南; ²湖南省中药粉体与创新药物省部共建国家重点实验室培育基地, 长沙 410208, 湖南;³湖南中医药大学第一附属医院，长沙 410007, 湖南

收稿日期:2016-09-21 修回日期:2016-11-03 出版日期:2017-04-26 发布日期:2017-04-26
通讯作者: 王宇红，女，博士生导师，研究员，研究方向为中药神经药理。 E-mail:wyh107@126.com
作者简介:孟盼，女，助教，研究方向为神经精神疾病的机制研究及防治。 E-mail:mengpan0822@163.com
基金资助:
国家自然科学基金青年项目(81403387)；湖南省教育厅一般项目（14C0862，15C1038）；湖南中医药大学校级青年基金(99820001-147)；中医内科学省部共建教育部重点实验室开放基金（ZYNK201503）

Effect of Baishile capsule on expressions of BDNF，NT-3，NGF in septo-temporal axis of hippocampus of depressive rats

MENG Pan^1,2, LIU Zhuo ^1,2, ZHU Qing^1,2, ZHAO Hongqing ^1,2, ZHANG Xiuli ^1,2, LEI Chang ^1,2, WANG Yuhong ^1,2, YANG Hui ³

¹Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan, China; ² Training Bases of Hunan Key Laboratory of Chinese Materia Medica Powder and Innovative Drugs Established by Provincial and Ministry, Changsha 410208, Hunan, China; ³ the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan, China

Received:2016-09-21 Revised:2016-11-03 Online:2017-04-26 Published:2017-04-26

摘要/Abstract

摘要：

目的: 探讨百事乐胶囊对慢性应激抑郁模型大鼠海马隔颞轴脑源性神经营养因子（BDNF）、神经营养因子3（NT-3）、神经生长因子（NGF）表达的影响。方法: 将60只SD大鼠随机分为空白对照组、抑郁模型组、氟西汀组、百事乐胶囊高、中、低剂量（2.88、1.44、0.72 g/kg）组，每组10只。采用慢性不可预见性温和应激建立抑郁大鼠模型，连续应激21 d后，采用糖水偏好实验和强迫游泳实验测试大鼠的抑郁样行为，Morris水迷宫测试评价大鼠的学习记忆能力，采用免疫荧光技术观察大鼠海马隔颞轴神经营养因子BDNF、NT-3、NGF蛋白表达的变化。结果: 与空白组比较，模型组大鼠糖水偏好率显著降低，游泳不动时间增加（P<0.01）；逃避潜伏期、目标象限潜伏时间显著增长（P<0.01），穿越目标象限的次数显著降低（P<0.05）；海马隔颞轴S1、S2、T3、T4部分BDNF、NT-3、NGF表达下调（P<0.01）。在给予百事乐胶囊干预后，模型大鼠的糖水偏好率显著增加，不动时间降低（P<0.05）；同时大鼠的逃避潜伏期及目标象限潜伏时间明显缩短（P<0.05），穿越目标象限的次数增加（P<0.05）；此外，百事乐胶囊也能使模型大鼠海马隔颞轴S2、T3部分BDNF、NT-3、NGF的表达显著增加（P<0.05或P<0.01）。结论: 百事乐胶囊能显著改善模型大鼠的抑郁样行为，提高其学习记忆能力，其作用可能与调节海马隔颞轴S2、T3部分的功能，促进海马神经营养因子的表达有关。

关键词: 百事乐胶囊, 抑郁, 慢性应激, 海马, 隔颞轴, 神经营养因子

Abstract:

AIM: To investigate the effect of Baishile capsule on the expression of neurotrophic factor including brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3), and nerve growth factor (NGF) in hippocampal septo-temporal axis of depressive rats. METHODS: Sixty SD rats were randomly divided into control group, model group of depression, fluoxetine group, high (2.88 g/kg), medium (1.44 g/kg) and low (0.72 g/kg) doses of Baishile capsule. Chronic unpredictable mild stress for 21 d was used to establish depression model; sucrose preferences and forced swimming test to detect depression-like behavior in rats; Morris water maze test to evaluate learning and memory ability; immunofluorescence technique to observe the content change of neurotrophic factors (BDNF,NT-3,NGF) in hippocampal septo-temporal axis of rats. RESULTS: Compared with the control group, sucrose preference was decreased while immobility time was increased significantly (P<0.01); escape latency and latency time in target quadrant presented a significant increase (P<0.01); times of crossing the target quadrant were significantly reduced (P<0.05); BDNF, NT-3, NGF expressions were down-regulated in S1, S2, T3 and T4 parts of hippocampal septo-temporal axis in model rats (P<0.01). Compared with model group, Baishile capsule could significantly improve sucrose preference degree and decrease immobility time (P<0.05); escape latency and latency time in target quadrant were significantly shorter (P<0.05); times of crossing the target quadrant increased in rats after administration of Baishile capsule (P<0.05); BDNF, NT-3, NGF expressions increased in S2 and T3 parts of septo-temporal axis in depressive rats (P<0.05 or P<0.01). CONCLUSION: Baishile capsule could significantly improve the depression-like behavior, learning and memory ability in depressive rats, which might be associated with regulating the function of S2, T3 parts of hippocampal septo-temporal axis, and promoting the expression of neurotrophic factors.

Key words: Baishile capsule, depression, chronic stress, hippocampus, septo-temporal axis, neurotrophic factor

中图分类号:

R965.2

孟盼，柳卓，朱青，赵洪庆，张秀丽，雷昌，王宇红，杨蕙. 百事乐胶囊对抑郁模型大鼠海马隔颞轴BDNF、NT-3、NGF表达的影响[J]. 中国临床药理学与治疗学, 2017, 22(4): 418-423.

MENG Pan, LIU Zhuo, ZHU Qing, ZHAO Hongqing, ZHANG Xiuli, LEI Chang, WANG Yuhong, YANG Hui. Effect of Baishile capsule on expressions of BDNF，NT-3，NGF in septo-temporal axis of hippocampus of depressive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(4): 418-423.

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