深II度烧伤皮肤miRNA差异表达研究

doi:10.12092/j.issn.1009-2501.2018.12.001

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (12): 1321-1328.doi: 10.12092/j.issn.1009-2501.2018.12.001

• 基础研究 • 下一篇

深II度烧伤皮肤miRNA差异表达研究

潘晓秋, 翟红军, 李丽晖, 班耀林

中国医科大学附属第一医院鞍山医院, 鞍山 114000, 辽宁

收稿日期:2018-06-26 修回日期:2018-11-20 出版日期:2018-12-26 发布日期:2018-12-27
通讯作者: 翟红军，女，主任医师，主要从事烧伤外科学研究。 E-mail:hongjunzhai@163.com
作者简介:潘晓秋,女，主管药师，主要从事临床药学工作。 E-mail:xiaoqiu912@126.com
基金资助:
国家高技术研究发展计划(863计划,SS2014AA020705)

Study on differential expression of the mi-RNA in the deep second degree burns

PAN Xiaoqiu, ZHAI Hongjun, LI Lihui, BAN Yaolin

Anshan Hospital, the First Affiliated Hospital of China Medical University, Anshan 114000, Liaoning, China

Received:2018-06-26 Revised:2018-11-20 Online:2018-12-26 Published:2018-12-27

摘要/Abstract

摘要：

目的: 研究深II度烧伤皮肤与正常皮肤组织中miRNA差异表达谱，探讨差异表达miRNAs在创面愈合中的作用。方法: 利用miRNA基因芯片检测技术筛选出深II度烧伤皮肤与正常皮肤组织差异表达的miRNA，运用实时定量RT-PCR验证部分miRNA表达情况。通过基因数据库TargetScan、miRanda和mirbase进行靶基因预测。对预测的靶基因利用GO-Analysis进行靶向性功能分析及Pathway进行信号通路分析。结果: 深II度组与正常组相比，上调的有41个，下调的有124个；其中miR-210-3P，miR-99a-5P，miR-99b-3P，miR-99b-5P，miR-143-5P，miR-29c-5P，miR-15b-5P，miR-323a-3P，miR-424-5P，miR-125b-1-3P，miR-125a-3P，miRNA-184差异表达显著，且参与创面炎症反应、免疫应答及愈合过程。结论: 在基因水平上确定差异表达的miRNAs及其对应靶基因在烧伤创面愈合中的调控机制，为烧伤创面精准治疗提供靶点。

关键词: 深II度烧伤, 皮肤, miRNA

Abstract:

AIM:To study the differential expression profile of the miRNA in deep second-degree burns and normal skin tissue, and to investigate the effects of differential expression of the miRNA on wound healing. METHODS: The differential expression of miRNA in deep second-degree burns and normal skin tissue were screened by gene chip detection technology, and the expression of some miRNA was verified by RT-PCR. Target gene prediction was carried out through gene databases TargetScan, miRanda and mirbase. The targeting function analysis and signaling pathway analysis for the predicted target genes were completed with the help of GO-analysis.RESULTS:Compared with the normal group, there were 41 miRNAs genes up-regulated and 124 miRNAs genes down-regulated in the deep second-degree group, and greater differences were shown in some genes, including miR-210-3P, miR-99a-5P, miR-99b-3P, miR-99b-5P, miR-143-5P, miR-29c-5P, miR-15b-5P, miR-323a-3P, miR-424-5P, miR-125b-1-3P, miR-125a-3P, miRNA-184, which were involved in the inflammatory response, immune response and the healing process. CONCLUSION:The regulatory mechanism of miRNA with differential expression and its corresponding target genes in the healing of burn wounds was confirmed at the gene level, which provided targets for the precise treatment for burn wounds.

Key words: the deep second-degree burns, skin, miRNA

中图分类号:

R965.2
R96

潘晓秋, 翟红军, 李丽晖, 班耀林. 深II度烧伤皮肤miRNA差异表达研究[J]. 中国临床药理学与治疗学, 2018, 23(12): 1321-1328.

PAN Xiaoqiu, ZHAI Hongjun, LI Lihui, BAN Yaolin. Study on differential expression of the mi-RNA in the deep second degree burns[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(12): 1321-1328.

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深II度烧伤皮肤miRNA差异表达研究

Study on differential expression of the mi-RNA in the deep second degree burns

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