左卡尼汀对大鼠糖尿病肾病的保护作用分析

doi:10.12092/j.issn.1009-2501.2019.03.007

中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (3): 278-282.doi: 10.12092/j.issn.1009-2501.2019.03.007

左卡尼汀对大鼠糖尿病肾病的保护作用分析

赵韶静¹，曾艳¹，李正东¹，张华²

¹湖北医药学院附属东风医院肾内科，²检验科，十堰 442008，湖北

收稿日期:2018-10-10 修回日期:2018-11-15 出版日期:2019-03-26 发布日期:2019-04-01
通讯作者: 曾艳，女，硕士研究生，主治医师，主要从事慢性肾脏病的诊治。 Tel：13636264972 E-mail：zengyan4927@163.com
作者简介:赵韶静，女，硕士研究生，主治医师，主要从事慢性肾脏病的诊治。 Tel：18986877160 E-mail：zhaosj31@163.com
基金资助:
2014年十堰市科学技术研究与开发项目（14Y54）

Protective effects of L-carnitine on diabetic nephropathy in rats

ZHAO Shaojing¹, ZENG Yan ¹, LI Zhengdong ¹, ZHANG Hua²

¹Department of Nephrology, Dongfeng Hospital, Hubei University of Medicine, Shiyan 442008, Hubei, China; ²Department of Clinical Laboratory, Dongfeng Hospital, Hubei University of Medicine, Shiyan 442008, Hubei, China

Received:2018-10-10 Revised:2018-11-15 Online:2019-03-26 Published:2019-04-01

摘要/Abstract

摘要：

目的: 探讨与分析左卡尼汀对大鼠糖尿病肾病的保护作用。方法: 24只8周龄的雄性SD大鼠随机分为3组，每组8只大鼠。为对照组提供单次腹腔内生理盐水(10 mL/kg)注射，接着静脉注射生理盐水(1 mL/kg)；为糖尿病肾病组提供单次腹腔内链脲霉素(65 mg/kg)注射，接着静脉注射生理盐水(1 mL/kg)；对于实验组，则提供单次腹腔内链脲霉素(65 mg/kg)注射，接着静脉注射左卡尼汀(50 mg/kg)，都为1次/d，持续14 d，记录实验后肾功能情况。结果: 实验组与糖尿病肾病组都造模成功，实验后实验组与对照组的体质量显著高于糖尿病肾病组(P<0.05)，血肌酐与尿蛋白排泄量及IL-6、TNF-α含量都显著低于糖尿病肾病组，肾组织Bcl-2、Caspase-3表达量都显著低于糖尿病肾病组，实验组与对照组之间对比差异无统计学意义(P>0.05)。结论: 左卡尼汀在大鼠糖尿病肾病中的应用能有效发挥肾功能保护作用，抑制肾组织的Bcl-2、Caspase-3表达，促进大鼠体质量恢复正常。

关键词: 左卡尼汀, 大鼠, 糖尿病肾病, 肾功能

Abstract:

AIM: To investigate and analysis the protective effects of L-carnitine on diabetic nephropathy in rats. METHODS: Twenty-four 8-week-old male SD rats were randomly divided into three groups of 8 rats each. The control group were received single intraperitoneal injection of normal saline (10 mL/kg), followed by intravenously injection of normal saline (1 mL/kg). The diabetic nephropathy group were received single intraperitoneal injection of streptozotocin (65 mg/kg), followed by intravenously injection of normal saline(1 mL/kg). The experimental group were received single intraperitoneal injection of streptozotocin (65 mg/kg), followed by intravenously injection of intravenous L-carnitine (50 mg/kg), 1 time/d for 14 d, and the renal function after the experiment were recorded.RESULTS:The models of diabetic nephropathy were successfully established both in the experimental group and the diabetic nephropathy group. After the experiment, the body weight of the experimental group and the control group were significantly higher than that of the diabetic nephropathy group (P<0.05). Serum creatinine and urinary protein excretion, IL-6 and TNF-α, Bcl-2 and Caspase-3 in renal tissue of the experimental group and the control group were significantly lower than those of diabetic nephropathy group (P<0.05). There was no significant difference between the experimental group and the control group (P>0.05).CONCLUSION: The application of L-carnitine in diabetic nephropathy can effectively protect renal function, inhibit the expression of Bcl-2 and Caspase-3 in kidney tissue, and promote the recovery of normal body weight.

Key words: L-carnitine, rat, diabetic nephropathy, renal function

中图分类号:

赵韶静，曾艳，李正东，张华. 左卡尼汀对大鼠糖尿病肾病的保护作用分析[J]. 中国临床药理学与治疗学, 2019, 24(3): 278-282.

ZHAO Shaojing, ZENG Yan, LI Zhengdong, ZHANG Hua. Protective effects of L-carnitine on diabetic nephropathy in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(3): 278-282.

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左卡尼汀对大鼠糖尿病肾病的保护作用分析

Protective effects of L-carnitine on diabetic nephropathy in rats

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