AIM: The purpose of this paper is to study whether the precise administration of vancomycin under the guidance of the model can achieve better clinical treatment effect by implementing the precise medication model in Sichuan Provincial People's Hospital, so as to promote its wide application in practice. METHODS: A prospective cohort study was conducted. The experimental group was patients who used the Model-informed Precision Dosing to determine the vancomycin dosing regimen, and the control group was patients who only decided the vancomycin dosing regimen based on the existing diagnosis and treatment process. Two sets of data were matched to compare whether there were significant differences between the two groups of patients in the TDM steady-state trough concentration or the steady-state AUC compliance ratio, and to evaluate the application value of the model software. RESULTS: According to the inclusion and exclusion criteria, the steady-state blood concentration data of vancomycin in a total of 280 patients were used for analysis. The final result of the experiment was that the number of people in the experimental group was 10, and the compliance rate was 62.50%. There were 64 cases in the control group, and the compliance rate was 24.24% (P=0.002). CONCLUSION: The study found that the model-guided precise dosing regimen can more accurately control the blood concentration of vancomycin in patients, and significantly improve the patient's steady-state trough concentration compliance rate. Therefore, it is recommended to increase the research on the program and promote its wide application in clinical practice.