IgD对类风湿关节炎患者外周血诱导的单核-巨噬细胞极化、吞噬的影响

doi:10.12092/j.issn.1009-2501.2026.04.002

中国临床药理学与治疗学 ›› 2026, Vol. 31 ›› Issue (4): 443-450.doi: 10.12092/j.issn.1009-2501.2026.04.002

IgD对类风湿关节炎患者外周血诱导的单核-巨噬细胞极化、吞噬的影响

陈梦琴¹(), 凌夕¹, 褚强强², 刘丹彦¹, 王越业¹, 吴甜甜¹, 董满玲¹, 魏伟¹, 吴育晶¹^,*()

1. 安徽医科大学药学科学学院临床药理研究所，抗炎免疫药物教育部重点实验室（安徽医科大学），合肥 230032，安徽
2. 合肥市第一人民医院，安徽医科大学第三附属医院，合肥 230061，安徽

收稿日期:2024-12-09 修回日期:2025-02-24 出版日期:2026-04-26 发布日期:2026-04-30
通讯作者: 吴育晶 E-mail:1729472397@qq.com;wyj@ahmu.edu.cn
作者简介:陈梦琴，女，硕士研究生，从事抗炎免疫药理学研究。E-mail：1729472397@qq.com
基金资助:
安徽省科技厅自然科学基金面上项目（2308085MH311）；安徽省教育厅自然科学基金重点项目（2023AH050666）；安徽医科大学第三附属医院基础与临床合作研究提升计划培育专项项目（2023sfy005）

Effects of IgD on polarization and phagocytosis of peripheral blood induced monocyte-macrophage in patients with rheumatoid arthritis

Mengqin CHEN¹(), Xi LING¹, Qiangqiang CHU², Danyan LIU¹, Yueye WANG¹, Tiantian WU¹, Manling DONG¹, Wei WEI¹, Yujing WU¹^,*()

1. Institute of Clinical Pharmacology, School of Pharmacy, Anhui Medical University; Key Laboratory of Anti-inflammatory and Immune Medicine (Anhui Medical University), Ministry of Education, Hefei 230032, Anhui, China
2. The Third Affiliated Hospital of Anhui Medical University, The First People's Hospital of Hefei, Hefei 230061, Anhui, China

Received:2024-12-09 Revised:2025-02-24 Online:2026-04-26 Published:2026-04-30
Contact: Yujing WU E-mail:1729472397@qq.com;wyj@ahmu.edu.cn

摘要/Abstract

摘要：

目的: 研究免疫球蛋白D（immunoglobulinD，IgD）对人外周血诱导的单核-巨噬细胞功能的影响。方法: 收集类风湿关节炎（rheumatoid arthritis，RA）患者和健康对照者静脉血，分离人外周血单个核细胞（peripheral blood mononuclear cells，PBMCs），贴壁后用巨噬细胞集落刺激因子（macrophage colony-stimulating factor，M-CSF）刺激，流式细胞术检测RA患者和健康对照者外周血单核-巨噬细胞表面IgD Fc受体（FcδR，IgDR）的表达。给予不同浓度的IgD（终浓度1、3、10 μg/mL）刺激48 h，流式细胞术检测单核-巨噬细胞表面CD86、CD206的表达；流式细胞术检测单核-巨噬细胞的吞噬能力。结果: 人外周血单核-巨噬细胞表面连续表达FcδR，相较于健康对照者，RA患者外周血的表达更高（P<0.01）。IgD可增加M1型巨噬细胞的百分比（P<0.01），同时，巨噬细胞的吞噬能力也随着IgD水平的升高而增强（P<0.01）。结论: IgD可能通过结合FcδR促进人外周血诱导的单核-巨噬细胞向M1型极化，引起 M1、M2型巨噬细胞比例的失衡，并增强巨噬细胞的吞噬能力。

关键词: 免疫球蛋白D, 类风湿关节炎, 巨噬细胞, 极化, 吞噬

Abstract:

AIM: To investigate the effects of immunoglobulin D (IgD) on the function of human peripheral blood macrophages. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from rheumatoid arthritis (RA) patients and healthy controls, and then adhered to the wall and stimulated with macrophage colony-stimulating factor (M-CSF). Flow cytometry was used to detect the expression of IgD Fc receptors (FcδR, IgDR) on the surface of peripheral blood mononuclear macrophages in RA patients and healthy controls. Stimulate macrophages with different concentrations of IgD (final concentrations of 1, 3, 10 μg/mL) for 48 hours, and flow cytometry was used to detect the expression of CD86 and CD206 on the surface of macrophages. Flow cytometry was used to detect the phagocytic of macrophages. RESULTS: Continuous expression of FcδR was detected on macrophages induced by peripheral blood mononuclear cells, and the expression was higher in RA patients than in healthy controls. The expression of M1 macrophages can be enhanced by IgD, while the expression of M2 macrophages can be weakened. At the same time, the phagocytic ability of macrophages are also enhanced with the increase of IgD levels. CONCLUSION: IgD may promote macrophage polarization in human peripheral blood monocytes by binding to FcδR, causing an imbalance in the proportion of M1 and M2 macrophages and enhancing phagocytic function of macrophages.

Key words: immunoglobulin D, rheumatoid arthritis, macrophage, polarization, phagocytosis

中图分类号:

陈梦琴, 凌夕, 褚强强, 刘丹彦, 王越业, 吴甜甜, 董满玲, 魏伟, 吴育晶. IgD对类风湿关节炎患者外周血诱导的单核-巨噬细胞极化、吞噬的影响[J]. 中国临床药理学与治疗学, 2026, 31(4): 443-450.

Mengqin CHEN, Xi LING, Qiangqiang CHU, Danyan LIU, Yueye WANG, Tiantian WU, Manling DONG, Wei WEI, Yujing WU. Effects of IgD on polarization and phagocytosis of peripheral blood induced monocyte-macrophage in patients with rheumatoid arthritis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2026, 31(4): 443-450.

图/表 5

图 1

Fig.1 Verify the percentage of monocyte-derived macrophages induced by M-CSF

图 2

Fig.2 Expression of FcδR on monocyte-derived macrophages in PBMCs of RA patients and healthy controls ($ \overline{x} $±s, n=3) A: expression of FcδR tested by flow cytometry; B: the statistical chart shows the expression of FcδR in RA patients and healthy controls. cP<0.01, compared with HC-FcδR group.

图 3

Fig.3 Effects of IgD on the phagocytic activity of mononuclear macrophages in PBMCs of RA patients and healthy controls A: circle CD68+ and FITC+ cell clusters in the cell population; B: fluorescence imaging of individual cells every group.

图 4

Fig.4 Effects of IgD on the expression of CD86 and CD206 in healthy controls PBMCs ($ \overline{x} $±s, n=5) A: expression of CD86 and CD206 tested by flow cytometry in PBMCs of healthy controls; B: the statistical chart shows the ratio of M1/M2 macrophages under different concentrations of IgD stimulation. cP<0.01, compared with control group.

图 5

Fig.5 Effects of IgD on the expression of CD86 and CD206 in RA patients PBMCs ($ \overline{x} $±s, n=5) A: expression of CD86 and CD206 tested by flow cytometry in PBMCs of RA patients; B: the statistical chart shows the ratio of M1/M2 macrophages under different concentrations of IgD stimulation. cP<0.01, compared with control group; C: comparison of RA patients and healthy controls of M1/M2 under different concentrations of IgD.

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IgD对类风湿关节炎患者外周血诱导的单核-巨噬细胞极化、吞噬的影响

Effects of IgD on polarization and phagocytosis of peripheral blood induced monocyte-macrophage in patients with rheumatoid arthritis

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