IL-17A与脂多糖诱导的小鼠急性肺损伤早期反应的关系

doi:10.12092/j.issn.1009-2501.2018.02.006

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (2): 148-153.doi: 10.12092/j.issn.1009-2501.2018.02.006

IL-17A与脂多糖诱导的小鼠急性肺损伤早期反应的关系

郑爽¹，付秦磊¹，周玲萍²，彭传鹏³，徐红蕾⁴

¹温州医科大学第一临床医学院，温州 325000，浙江； ²温州医科大学附属第一医院呼吸内科，温州 325000，浙江；³温州医科大学附属第三医院老年病科，温州 325000，浙江；⁴温州医科大学附属第一医院急诊医学科，温州 325000，浙江

收稿日期:2017-06-19 修回日期:2017-10-09 出版日期:2018-02-26 发布日期:2018-03-02
通讯作者: 徐红蕾，女，硕士，教授，硕士生导师，研究方向：急性肺损伤。 Tel：0577-55579271 E-mail：xfhl2000@163.com
作者简介:郑爽，女，硕士研究生，研究方向：呼吸内科。 Tel：15067896753 E-mail：470559276@qq.com
基金资助:
温州市科技局科研基金（Y20140680）；浙江省卫生厅科研基金（2015RCB018）；浙江省自然科学基金（LY12H01001）

Study on interleukin-17A during early inflammatory response in mice with LPS-induced acute lung injury

ZHENG Shuang ¹, FU Qinlei ¹, ZHOU Lingping², PENG Chuanpeng³, XU Honglei⁴

¹ The First Clinical Medical College, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China; ² Department of Respiratory Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China; ³ Geriatric Department, the Third Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China; ⁴Emergency Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China

Received:2017-06-19 Revised:2017-10-09 Online:2018-02-26 Published:2018-03-02

摘要/Abstract

摘要：

目的: 观察肺泡灌洗液中白介素17A（IL-17A）在脂多糖（LPS）诱导的急性肺损伤（ALI）小鼠中的变化，初步探讨其与ALI早期炎症反应的关系。方法: 气管注射脂多糖（10 mg/kg），建立ALI模型组，对照组予等量生理盐水，在 3、8、14、24 h四个时间点处死，ELISA检测支气管肺泡灌注液（BALF）中IL-17A及血清中的单核细胞趋化蛋白1（MCP-1）水平，肺组织病理形态变化，肺湿重/干重比值（W/D），Western blot分析肺组织中细胞间粘附分子1（ICAM-1）表达，比色法测定肺组织髓过氧化物酶（MPO）活力。结果: 与对照组相比，ALI组早期BALF的IL-17A水平增加（P<0.05），伴血清MCP-1水平急剧升高（P<0.01），随着造模时间的延长，ALI组病理HE染色有明显炎症改变，W/D、ICAM-1表达、MPO活力均显著上升（均P<0.05）。结论: 急性肺损伤早期支气管灌洗液中的IL-17A明显上升，IL-17A在急性肺损伤的早期炎症反应的发生发展中可能有重要的相关性。

关键词: 急性肺损伤, 脂多糖, 白介素17A

Abstract:

AIM: To observe the dynamic changes of IL-17A in lipopolysaccharide (LPS)-induced acute lung injury(ALI) in mice, and to assess the correlation of IL-17A during early inflammatory response of ALI. METHODS: Mice were used to establish the ALI group and its control group by intratracheal administration of LPS and normal saline, respectively. The level of IL-17A in bronchoalveolar lavage fluid (BALF) and MCP-1 in serum, histopathological changes in lung tissue, lung wet weight /dry weight ratio (W/D), the expression of intercellular adhesion molecule 1 (ICAM-1) and the myeloperoxidase (MPO) activity in lung homogenates would be tested after modeling for 3, 8, 14, 24 h. RESULTS: Compared with the controls, the level of IL-17A in BALF increased (P<0.05) and the level of MCP-1 in serum increased significantly (P<0.01), and as time went by, the three above decreased gradually, and the histological examination of lung showed inflammatory changes, and W/D ratio, expression of ICAM-1, MPO activity aggravated gradually in the ALI group (P<0.05). CONCLUSION: The level of IL-17A in BALF increased significantly during early ALI, and this shows that IL-17A may play an important role in the progress of early inflammatory response in LPS-induced ALI.

Key words: acute lung injury, lipopolysaccharide, IL-17A

中图分类号:

R965.2

郑爽，付秦磊，周玲萍，彭传鹏，徐红蕾. IL-17A与脂多糖诱导的小鼠急性肺损伤早期反应的关系[J]. 中国临床药理学与治疗学, 2018, 23(2): 148-153.

ZHENG Shuang, FU Qinlei, ZHOU Lingping, PENG Chuanpeng, XU Honglei. Study on interleukin-17A during early inflammatory response in mice with LPS-induced acute lung injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(2): 148-153.

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IL-17A与脂多糖诱导的小鼠急性肺损伤早期反应的关系

Study on interleukin-17A during early inflammatory response in mice with LPS-induced acute lung injury

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