关键词: 复方黄栌口服液, 大鼠, 慢性肝损伤模型, 护肝宁片

Abstract:

AIM: To observe the effects of compound Cotinus coggygria oral liquid on ALT, AST, SOD, MDA, Hyp levels of liver homogenate and liver tissue in rats with chronic liver injury induced by carbon tetrachloride. METHODS: Seventy-two male SD rats, half male and half female, were randomly divided into blank group, model group, Huganing tablet group (0.675 g/kg), high, medium and low dose of compound Cotinus coggygria oral liquid group (15, 10, 5 mL/kg, respectively). Rat model of chronic liver injury was induced by intraperitoneal injection of 50% carbon tetrachloride olive oil solution 1 mL/kg, 2 times/week for 8 weeks, and assigned drugs were administered at fifth weeks at the same time accordingly. Twenty-four hours after the final administration, rats were anesthetized by intraperitoneal injection of chloral hydrate, and the inferior vena cava blood was collected to detect the levels of AST, ALT, MDA, Hyp, and SOD in liver homogenate. Also, a small amount of liver tissue was collected to observe the pathological changes. RESULTS: Rat model of chronic liver injury model was successfully induced. Compared with the model group, high, medium and low dose of compound Cotinus coggygria oral liquid and Huganning tablet could significantly reduce the ALT, AST and MDA level (P<0.01), increase SOD level (P<0.01) , lower Hyp levels (P<0.01, P<0.05) in liver homogenate, and could significantly improve liver pathological damages of model rats (P<0.01). CONCLUSION: Compound Cotinus coggygria oral liquid has a better protective effect on chronic liver injury induced by CCl₄.

Key words: compound Cotinus coggygria oral liquid, rats, chronic liver injury model, Huganning tablet