欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (5): 517-521.

• 基础研究 • 上一篇    下一篇

碳青霉烯类耐药肺炎克雷伯菌临床分离株中质粒介导的喹诺酮类耐药基因检测

杭亚平, 宁长秀, 汪红, 钟桥石, 胡晓彦, 章白苓, 张楠, 胡龙华   

  1. 南昌大学第二附属医院检验科,南昌 330006,江西
  • 收稿日期:2013-11-30 修回日期:2014-05-09 出版日期:2014-05-26 发布日期:2014-06-05
  • 通讯作者: 胡龙华,主任技师,硕士生导师,研究方向:临床微生物学及细菌耐药机制。Tel: 0791-86300410 E-mail: longhuahu@163.com
  • 作者简介:杭亚平,女,在读硕士研究生,研究方向:临床微生物学及细菌耐药机制。Tel: 15870647593 E-mail:bingbinghang@hotmail.com.com
  • 基金资助:
    江西省科技厅科技支撑计划(20112BBG70054)

The detection of plasmid mediated quinolone resistance genes in carbapenem-resistant klebsiella-pneumoniae clinical isolates

HANG Ya-ping, NING Chang-xiu, WANG Hong, ZHONG Qiao-shi, HU Xiao-yan, ZHANG Bai-ling, ZHANG Nan, HU Long-hua   

  1. Department of Laboratory Medicine,the Second Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi,China
  • Received:2013-11-30 Revised:2014-05-09 Online:2014-05-26 Published:2014-06-05

摘要: 目的:探讨质粒介导的喹诺酮类耐药(PMQR)基因在碳青霉烯类耐药肺炎克雷伯菌 (CRKP)临床分离株中的分布情况。方法:收集CRKP 29株,聚合酶链反应(PCR)扩增并确定产物基因型。结果:CRKP中PMQR基因检出率为48.3%(14/29),其中qnrB 5株,包括qnrB2 1株、qnrB4 3株、qnrB10 1株;qnrS 5株,均为qnrS1;1株同时携带 qnrS1和qnrB4;有3株携带aac(6')-Ib-cr基因。所有PMQR基因阳性菌株均同时携带β-内酰胺酶基因,其中8株同时携带碳青霉烯酶基因,占 57.1%(8/14),以 blaKPC-2(4/14)及blaNDM-1(3/14)为主。结论:PMQR基因在临床分离的CRKP中较为普遍,以qnr基因为主,且qnr阳性菌株碳青霉烯酶基因携带率较高,均为多重耐药株。

关键词: 质粒介导的喹诺酮耐药基因, 耐药, 碳青霉烯类耐药, 肺炎克雷伯菌

Abstract: AIM: To investigate distribution of plasmid mediated quinolone resistance(PMQR) genes in carbapenem-resistant klebsiella-pneumoniae(CRKP) clinical isolates.METHODS: 29 CRKPs were collected , PCR amplifications were performed and the products were sequenced to determine the genetypes.RESULTS: The detection rate of PMQR genes was 48.3%(14/29),5 strains carring qnrB gene, including qnrB2 1 strain,qnrB4 3 strain,qnrB10 1 strain; 5 strains carring qnrS1 gene; 1 strain both carring qnrS1 and qnrB4; 3 strains carring aac(6')-Ib-cr gene. All the 14 strains were positive for PMQR genes harbored β-lactamase genes and 8 strains also harbored carbapenemase genes, accounting for 57.1%(8/14),blaKPC-2(4/14)and blaNDM-1(3/14)were the main genotypes of carbapenemase genes.CONCLUSION: PMQR genes are common among the clinical isolates of CRKP, predominantly qnr genotype,and the carring rate of carbapenemase genes is high in qnr-positive isolates,all which are multiple drug-resistant strains.

Key words: plasmid mediated quinolone resistance gene, drug resistance, carbapenem-resistance, klebsiella-pneumoniae

中图分类号: