Effects of volatile oil of the angelica on the proliferation and apoptosis of human lung adenocarcinoma cell GLC-82

doi:10.12092/j.issn.1009-2501.2018.01.006

Abstract

Abstract:

AIM: To investigate the cytotoxic effect and apoptosis of volatile oil of the angelica on human lung adenocarcinoma cell GLC-82. METHODS: MTT colorimetric assay was used to investigate the cytotoxic effect of human lung adenocarcinoma cell GLC-82. The cell apoptosis was analyzed by flow cytometry. AO/BE fluorescent staining was applied to observe the cell apoptosis. RESULTS: The GLC-82 cell was inhibited by volatile oil of the angelica in a concentration-dependent manner; the IC50 was 113.03 μg/mL. The result of flow cytometry indicated volatile oil of the angelica could induce apoptosis and necrosis of GLC-82 cell, and the rates of apoptosis and necrosis were increased by the increase of drug concentration. The results of AO/BE fluorescent staining method indicated the rate of apoptosis and necrosis increased in drug group along with the increase of drug concentration. CONCLUSION: Volatile oil can induce the apoptosis of GLC-82 cell.

Key words: volatile oil of the angelica, human lung adenocarcinoma cell GLC-82, apoptosis

CLC Number:

R965.2

ZHANG Yanxia, LIN Xingyao, AN Fangyu, ZHANG Zhengshun, CHEN Shaoyi, ZHOU Jiawei, ZHANG Yanying. Effects of volatile oil of the angelica on the proliferation and apoptosis of human lung adenocarcinoma cell GLC-82[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 29-33.

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