Methamphetamine dependent influenced RGS4 and mGluR5 receptor signaling in striatum of rats

doi:10.12092/j.issn.1009-2501.2018.05.009

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2018, Vol. 23 ›› Issue (5): 536-540.doi: 10.12092/j.issn.1009-2501.2018.05.009

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Methamphetamine dependent influenced RGS4 and mGluR5 receptor signaling in striatum of rats

ZHAO Linbo ^1,2，SUI Nianhan²，LI Lihua³，HONG Shijun³, XING Yuming ⁴, ZHAO Yongna ²

¹ School of Institute of Information Technology, Yunnan University Of TCM， Kunming 650500，Yunnan, China；² School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Product，Kunming Medical University，Kunming 650500，Yunnan, China； ³School of Forensic Medicine，Kunming Medical University，Kunming 650500，Yunnan, China；⁴ Key Laboratory of Narcotics Technology and Ministry of Police of Yunnan Provincial Police Department，Kunming 650228，Yunnan, China

Received:2017-11-17 Revised:2018-01-03 Online:2018-05-26 Published:2018-05-16

Abstract

Abstract:

AIM: To investigate whether regulator of G-protein signaling 4(RGS4 ) and metabotropic receptor 5 (mGluR5) expression level changed in striatum of the methamphetamine（METH）-dependent rat and whether the changes were related to the time of METH treatment. METHODS: METH-dependent rat model of one week and two weeks were established. The expression levels of RGS4, mGluR5, Galfaq, PLCbeta1 were detected by Western blot analysis. RESULTS: Rats treated with METH of one week and two weeks spent much more time in the METH-paired chamber than NS control group, suggested that METH-dependent model has successfully established. Compared with NS control group, the expression level of RGS4 in the striatum of rats in model group was down-regulated remarkably. Furthermore, the expression level of mGluR5 and its coupling protein Galfaq, phospholipase C-beta1(PLCbeta1) were up-regulated in different degrees when compared with NS control group, and the change is more remarkable in two weeks group than that of one week group. CONCLUSION: METH treatment down regulate the RGS4 and up regulate mGluR5 in rat striatum, and the expression level of mGluR5 coupling protein Galfaq, PLCbeta. And the longer the time of METH-dependent, the more significant the changes of RGS4, mGluR5, Galfaq, and PLCbeta1.

Key words: methamphetamine, striatum, CPP, RGS4, mGluR5, Galfaq, PLCbeta1

CLC Number:

R971

ZHAO Linbo，SUI Nianhan，LI Lihua，HONG Shijun, XING Yuming, ZHAO Yongna. Methamphetamine dependent influenced RGS4 and mGluR5 receptor signaling in striatum of rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(5): 536-540.

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Methamphetamine dependent influenced RGS4 and mGluR5 receptor signaling in striatum of rats

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