Yunnan Baiyao ameliorates MIA-induced knee osteoarthritis pain in rats through anti-inflammatory effect

doi:10.12092/j.issn.1009-2501.2019.03.003

Abstract

Abstract:

AIM: To study the mechanism of Yunnan Baiyao on MIA-induced knee osteoarthritis pain of rats. METHODS: Sprague-Dawley male rats (160-220) g were randomly divided into 4 groups: Normal group (B), Control group (C) ,Yunnan Baiyao group (YB, 125 mg/kg) and Diclofenac sodium group (DF, 2.5 mg/kg). Then they were given single intra-articular injection with 2 mg MIA through the infrapatellar ligament of the right knee except for the normal group (B). The Paw withdrawal threshold (PWT) and cold pain latency of the right hind limb of each rats were collected as the baseline the day before making the animal model, followed by measuring the PWT and cold pain latency twice a week. The serum PGE2 level was determined by ELISA 35 days after administration, and the knee joint sections were observed via the microscope after H&E staining. Evaluation of drugs was estimated according to the Markin's score and the data was analyzed by SPSS18.0. RESULTS: Compared with the blank group, the mechanical pain threshold of the model group was significantly reduced (P<0.01), and the level of PGE2 in serum was increased (P<0.01). Compared with the model group, Yunnan Baiyao and diclofenac sodium can significantly increase the mechanical pain threshold of knee osteoarthritis in rats (P<0.01), reduce the serum PGE2 level of knee osteoarthritis in rats (P<0.05), and delay the cartilage injury of knee osteoarthritis.CONCLUSION:Yunnan Baiyao possibly relieves the pain of knee osteoarthritis induced by MIA through anti-inflammation.

Key words: Yunnan Baiyao, sodium iodoacetate, knee osteoarthritis, pain, anti-inflammation

CLC Number:

R965.2

HU Peipei, HUANG Fang. Yunnan Baiyao ameliorates MIA-induced knee osteoarthritis pain in rats through anti-inflammatory effect[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(3): 254-259.

References

［1］郑伟康, 张宏, 王旭昀,等.中医药治疗膝骨关节炎研究进展［J］. 山西医药杂志, 2018，47(9):1013-1016.
［2］吕苏梅, 张瑞丽. 中老年膝骨关节炎的流行病学研究进展［J］. 中国老年学, 2016, 36(16):4133-4135.
［3］王斌, 邢丹, 董圣杰,等. 中国膝骨关节炎流行病学和疾病负担的系统评价［J］. 中国循证医学杂志, 2018，18(2):134-142.
［4］许松涛, 于之明, 周炳怀. 膝关节镜治疗膝骨性关节炎患者疗效的影响因素研究［J］. 安徽医药, 2014，18(6):1114-1116.
［5］He H, Ren X, Wang X, et al. Therapeutic effect of Yunnan Baiyao on rheumatoid arthritis was partially due to regulating arachidonic acid metabolism in osteoblasts［J］. J Pharm Biomed Anal，2012, 59：130-137.
［6］何夏秀, 曹炜, 冯兴华. 云南白药酊治疗膝骨关节炎30例临床总结［J］. 中国中医药信息杂志, 2003, 10(11):45-46.
［7］罗坚, 王祥华. 全膝关节置换联合云南白药治疗膝关节骨性关节炎的疗效及对ESR、血清CRP、MMP-9水平的影响［J］. 中国老年学杂志, 2017, 37(9)：2247-2248.
［8］Guingamp C, Gegout-Pottie P, Philippe L, et al. Mono-iodoacetate-induced experimental osteoarthritis. A dose-response study of loss of mobility, morphology, and biochemistry［J］. Arthritis & Rheumatism, 2010, 40(9):1670-1679.
［9］Fernihough J, Gentry C, Malcangio M, et al. Pain related behaviour in two models of osteoarthritis in the rat knee［J］. Pain, 2004, 112(1/2):83-93.
［10］Brenner DS, Golden JP, Vogt SK, et al. A simple and inexpensive method for determining cold sensitivity and adaptation in mice［J］. J Vis Exp, 2015, 2015，(97):e52640-e52640.
［11］Pitcher T , Sousa-Valente J , Malcangio M.The monoiodoacetate model of osteoarthritis pain in the mouse［J］. J Vis Exp，2016 May 16;(111). doi: 10.3791/53746.
［12］杜明泽, 段然, 邵德成, 等. 钾通道开放剂对大鼠碘乙酸钠所致骨关节炎疼痛作用的实验观察［J］. 中华医学杂志, 2017, 97(17):1333-1336.
［13］李姗. 超声导入复方三七消痛软膏对兔膝骨性关节炎软骨细胞caspase-3、caspase-9及XIAP表达的影响［D］. 福建中医药大学, 2016.
［14］Ryu J, Treadwell BV, Mankin HJ.Biochemical and metabolic abnormalities in normal and osteoarthritic human articular cartilage［J］. Arthritis & Rheumatology, 1984, 27(1):49-57.
［15］卢祺炯, 夏丽娟, 闫俊岭,等. 云南白药的一般药理学研究［J］. 中华中医药学刊, 2014,32(8):1879-1881.
［16］王雨来, 杜国安, 刘邦强.云南白药现代医学应用概述［J］. 时珍国医国药, 2006, 17(12):2580-2582.
［17］Wang T, Wan D, Shao L, et al. Notoginsenoside R1 stimulates osteogenic function in primary osteoblasts via estrogen receptor signaling［J］. Biochem Biophys Res Commun，2015, 466(2):232-239.
［18］卢伟, 牟雄军, 杨光义,等.中药重楼药理活性研究进展［J］. 中国药师, 2017, 20(5):896-899.
［19］董万超, 赵伟刚, 刘春华. 麝香研究进展［J］. 特产研究, 2001, 23(2):48-58.
［20］陈文学, 于德伟, 杨铭,等. 三七活血片抗软组织损伤、镇痛与抗炎药理作用研究［J］. 中国药房, 2015,26(25):3482-3484.
［21］Yan Y, Singh GK, Zhang F, et al.Comparative study of normal and rheumatoid arthritis fibroblast-like synoviocytes proliferation under cyclic mechanical stretch: role of prostaglandin E2［J］.Connect Tissue Res，2012，53(3):246-254.

[1]	ZHAO Lu, LI Fuyong, WEN Binhong, LI Daowei. Effects of stains on hyperlipidemia and pain relieve of patients with chronic migraine [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 671-675.
[2]	Qin LiYang. Effects of rapamycin intervention during pregnancy on cognitive function of autism model in rat offspring [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 841-847.
[3]	LIANG Hongyu, ZHANG Hailin, YIN Huanxin, CHEN Fan, LU Junlin, ZHU Caihong, HU Wei, ZHOU Renpeng. Effects of 2-APB in skin wound healing in mice through down-regulation of TRPM7 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(7): 747-753.
[4]	JIA Tao, TENG Jinliang. New narcotic analgesics: esketamine [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(7): 834-840.
[5]	SHEN Yanping, YIN Lijun, ZHUANG Wenming, YAN Haiya. Effective dose of esketamine for prevention on propofol injection pain in painless abortion [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 660-664.
[6]	CHEN Yugang, TU Yanqiong, WANG Congqing, CHEN Juan, ZHANG Bohong. Network pharmacology and molecular docking to discuss the mechanism of Jinhutongdan prescription in the treatment of cholelithiasis and experimental verification [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(10): 1090-1098.
[7]	WANG Rui, PAN Jinjin, LI Hua, YUAN Yuhui. Research progress of melatonin on pulmonary hypertension [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(4): 444-448.
[8]	ZHOU Haihui, ZHANG Haixia, GE Weihong. Analysis of the role of clinical pharmacist in the establishment of standardized cancer pain management ward [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(9): 1038-1042.
[9]	YANG Liqin, LI Zhiyu, TAN Pei, XU Wenting, ZHANG Zhongnan, WANG Mengya. Timed dose-response relationship of pain in autistic model rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1341-1346.
[10]	HUANG Lijia, HUANG Yang, KONG Jinsong, ZHENG Xin, GONG Xiaokang, RUAN Jianwei, WANG Haibao. Inhibition of articular cartilage degeneration by atorvastatin calcium via STAT1-caspase-3 signaling axis in rats with knee osteoarthritis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(7): 743-748.
[11]	FAN Yimin, WANG Limin, GUO Xiaoshu, FENG Gaizhuang, LI Xiuping. Lumbar plexus block with Ropivacaine for hind limbs pain of chronic ischemia in rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(4): 400-405.
[12]	MAO Hongxia, JIN Lingqing, LIU Shuangchun. Effect of glucosamine sulfate combined with Shenmai injection on knee osteoarthritis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(5): 560-564.
[13]	LIU Wencheng, TAN Buzhen，FANG Yuting, ZHAN Xinlu, WANG Yu. Progress of the study of anticancer and anti-inflammatory bioactive compounds of Tripterygium wilfordii [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(3): 355-360.
[14]	XU Xiangwei, CHEN Yanqiao, ZHU Peizhen. Therapeutic effect of amtiriptyline combined with opioids for moderate to severe cancer pain patients with depression [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(10): 1145-1151.
[15]	FEI Xianming, WANG Huan, YUAN Wufeng, JIANG Lei, CHENG Maoliang. Effects and preliminary molecular mechanisms of papain on the inhibition of monocyte secretory and adherent ability induced by monocyte-platelet aggregates [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(1): 1-8.