Expression and mechanism of p38MAPK signaling pathway in chronic pulpitis rats with pain

doi:10.12092/j.issn.1009-2501.2019.06.007

Abstract

Abstract:

AIM: To investigate the expression of p38MAPK signaling pathway in chronic pulpitis pain rats and its mechanism of action. METHODS: Twenty-five Wistar male rats of SPF grade were selected and divided into 3 groups, 5 in normal group, 15 in model group and 5 in MAPK inhibitor group. In the MAPK inhibitor group and the model group, the model of pulpitis was prepared. After successful modeling, the MAPK inhibitor group was injected with SB203580 at a dose of 5 mg/kg from the jugular vein for 14 days. The MAPK inhibitor group was selected 14 days after modeling. Five rats in the model group were sacrificed at 3, 7 and 14 d after modeling, respectively. Their pulp tissue pathology, serum interleukin-6 (IL-6), IL-8 content, p-p38MAPK, ERK, p-ERK, p38MAPK protein expression and p38MAPK, ERK mRNA expression were observed.RESULTS:Compared with the normal group, the length of apical damage, the width of the periapical periodontal membrane and the root necrosis rate of the first molar were significantly increased at 3, 7 and 14 days after modeling; while those in the MAPK inhibitor group were significantly lower (P<0.05). The levels of serum IL-6 and IL-8 in rats at 3, 7 and 14 days after modeling were significantly increased. Compared with 3, 7 and 14 days after modeling, serum IL-6 and IL-8 levels in rats with MAPK inhibitor group were significantly lower (P<0.05). Compared with the normal group, the expression of p-p38MAPK and p-ERK protein in the pulp tissue of the model group was increased. Compared with the model group, the MAPK inhibitor group The expression of p-p38MAPK and p-ERK protein in rat dental pulp tissue decreased (P<0.05).CONCLUSION: Activation of p38MAPK signaling pathway in dental pulp tissue of rats with chronic pulpitis increases the degree of inflammatory response, promotes disease progression, and inhibits p38MAPK signaling pathway, which can effectively reduce the expression of inflammatory factors.

Key words: pulpitis, p38MAPK signaling pathway, inflammatory cytokines

CLC Number:

R965.2

LI Min, WANG Shanshan, LIU Zhonghe, JIANG Deguo. Expression and mechanism of p38MAPK signaling pathway in chronic pulpitis rats with pain[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 644-649.

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