Effects of intrauterine perfusion of growth hormone on thin endometrium

doi:10.12092/j.issn.1009-2501.2019.09.004

Abstract

Abstract:

AIM: To study the therapeutic effect of intrauterine perfusion of growth hormone on thin endometrium. METHODS: Thirty female SD rats were randomly divided into three groups: control group, model group and GH group. The control group was not treated and routinely reared. The model group and GH group were perfused with 95% ethanol during estrus to establish a thin endometrial model. Six to eight hours after modeling, the model group was perfused with 0.2 mL normal saline, and the GH group was perfused with 0.2 mL growth hormone. HE staining was used to observe the thickness of endometrium, the number of blood vessels and glands. The expression of keratin and vimentin in rat endometrium was detected by immunoblotting and immunohistochemistry. RESULTS:The intima thickness, endometrial gland and intimal vessels in the GH group were increased compared with the model group (P<0.01). The intimal thickness, endometrial gland and intimal vessels were increased in the control group compared with the model group (P<0.01). There was no significant difference in intimal thickness, intimal glandular and intimal vessels between the control group and the GH group. The expression of vimentin and keratin in the GH group was increased (P<0.01). The expression of vimentin and keratin in the control group was increased (P<0.01). There was no significant difference in the expression of vimentin and keratin between the control group and the GH group. CONCLUSION:Intrauterine perfusion of growth hormone can promote the regeneration and repair of thin endometrium in rats.

Key words: growth hormone, intrauterine perfusion, thin endometrium, animal model

CLC Number:

R711

CAO Zhiwen, HU Weihua. Effects of intrauterine perfusion of growth hormone on thin endometrium[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(9): 983-988.

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