Compound Qishao Jiangya tablet reduces blood pressure in mesenteric artery of SHR rats by decreasing the deposition of type Ⅰ and type III collagen

doi:10.12092/j.issn.1009-2501.2019.12.008

Abstract

Abstract:

AIM: To explore the mechanism of compound Qishao Jiangya tablet on blood pressure and vascular remodeling in spontaneously hypertensive rats (SHR). METHODS: SHR rats were randomly divided into model group, irbesartan tablet group and compound Qishao Jiangya tablet group, 10 rats in each group; Another 10 WKY rats were used as normal control group; All received administration for 6 weeks; noninvasive sphygmomanometer was used to measure the changes of blood pressure in rats; HE staining was used to observe the pathological changes of mesenteric artery in rats and to measure the thickness of mesenteric artery wall; The thickness of intima media and the diameter of lumen in the same position were recorded as WT; Detection of matrix metalloprotease-2 (MMP-2) and type Ⅰ and type III collagen (CO Ⅰ, CO III) protein expression in mesenteric artery was performed by immuno-histochemical method. RESULTS:After 6 weeks of administration, compared with the normal control group, the blood pressure of the model group was significantly higher than that of the control group (P<0.01), and the thickness of tube wall and WT value were increased (P<0.01 or P<0.05), the expression of MMP -2, I and III collagen in mesenteric artery increased significantly (P<0.01); Compared with model group, the blood pressure of the rats in the compound Qishao Jiangya tablet group was significantly lower than that in the control group (P<0.01), The thickness of tube wall and WT value decreased (P<0.05), the expression of MMP-2 and type Ⅰ and type III collagen in mesenteric artery was significantly decreased (P<0.05), and the pathological changes were improved. CONCLUSION: Compound Qishaojiang tablet can reduce blood pressure and improve vascular remodeling in SHR rats, the mechanism may be related to the decrease of MMP-2 expression and the deposition of type Ⅰ and type III collagen.

Key words: compound Qishao Jiangya tablet, mesenteric artery, matrix metalloprotease-2, type Ⅰ collagen, type III collagen, vascular remodeling

CLC Number:

R965.2
R544

LI Hong, LIU Yeqian, GONG Shan, LIU Lin, ZENG Yong, CAI Xiao, WANG Yuhong, REN Weiqiong. Compound Qishao Jiangya tablet reduces blood pressure in mesenteric artery of SHR rats by decreasing the deposition of type Ⅰ and type III collagen[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1364-1370.

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