Effect of miR-423-5p on the injury of AR42J cells induced by caerulein through targeting AQP1

doi:10.12092/j.issn.1009-2501.2019.12.012

Abstract

Abstract:

AIM: To investigate the effect of miR-423-5p on the injury of pancreatic AR42J cells induced by caerulein and explore the underlying molecular mechanism. METHODS: A model of pancreatitis injury was established by treating AR42J cells with caerulein. The expression level of miR-423-5p in AR42J cells was detected by qRT-PCR. The expression levels of aquaporin 1(AQP1), Bcl-2, Bax and capase3 were measured by Western blot. The levels of IL-6 and TNF-α in culture supernatants were detected by enzyme-linked immunosorbent assay (ELISA). The cell apoptotic rate was determined by flow cytometry. The relationship between miR-423-5p and AQP1 was verified by dual-luciferase reporter assay system. RESULTS:miR-423-5p was up-regulated and AQP1 was down-regulated in AR42J cells induced by caerulein. Inhibition of miR-423-5p or overexpression of AQP1 alleviated the injury of AR42J cells induced by caerulein and inhibited cell apoptosis. The results of dual-luciferase reporter assay system suggested that miR-423-5p targeted and negatively regulated the expression of AQP1. Inhibition of AQP1 reversed the effects of inhibition of miR-423-5p on the injury of AR42J cells induced by caerulein. CONCLUSION: miR-423-5p alleviates the injury of pancreatic AR42J cells induced by caerulein and inhibited apoptosis by targeting AQP1.

Key words: pancreatitis, caerulein, miR-423-5p, aquaporin 1, AR42J cell injury, apoptosis

CLC Number:

R965.2

LIAO Wei, TANG Yucheng, ZHOU Tingting, ZHOU Furong. Effect of miR-423-5p on the injury of AR42J cells induced by caerulein through targeting AQP1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1394-1401.

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