Segmental absorption of helicid in rat everted intestinal sac model: A preliminary study

doi:10.12092/j.issn.1009-2501.2021.03.003

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2021, Vol. 26 ›› Issue (3): 258-263.doi: 10.12092/j.issn.1009-2501.2021.03.003

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Segmental absorption of helicid in rat everted intestinal sac model: A preliminary study

WANG Ping1, SHEN Jie2, CHU Jiru3, GUO Nan1, XIE Haitang1, ZHAN Cuijiao1, WANG Changmao1, YANG Bin2, ZHENG Dandan3, HU Rongfeng4, JIA Yuanwei1,2

¹ Pharmacy College, Wannan Medical College, Wuhu 241001, Anhui, China; ² Drug Clinical Evaluation Center of Anhui Province, Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China; ³Department of Pharmacy, Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China; ⁴ Key Laboratory of Xin'an Medicine, Ministry of Education, Hefei 230000, Anhui, China

Received:2020-08-11 Revised:2021-03-08 Online:2021-03-26 Published:2021-04-06

Abstract

Abstract: AIM: To investigate the absorption of helicid in different segments of intestine based on rat everted intestine sac model. METHODS: To establish a high-performance liquid chromatography method for simultaneous determination of helicid and its metabolite. Krebs-ringer solution containing helicid was added to everted intestine sacs of different segments (duodenum, Jejunum, ileum and colon). Drug concentration in sacs was determined at different time points (5, 10, 15, 30, 45, 60, 75, 90 min). Adsorptions of helicid in four intestinal segments were compared. RESULTS: This high-performance liquid chromatography was successfully applied to the simultaneous determination of helicid and its metabolite. Absorption of helicid was rapid and time-dependent. The absorption and metabolism of helicid in duodenum segment were higher than these in other segments. CONCLUSION: The duodenum segment is the main site of segmental absorption and metabolism of helicid. This is the first report on intestinal segment metabolism of helicid.

Key words: helicid, everted intestinal sac model, absorption, metabolism

CLC Number:

R965.2

WANG Ping, SHEN Jie, CHU Jiru, GUO Nan, XIE Haitang, ZHAN Cuijiao, WANG Changmao, YANG Bin, ZHENG Dandan, HU Rongfeng, JIA Yuanwei. Segmental absorption of helicid in rat everted intestinal sac model: A preliminary study[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(3): 258-263.

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Segmental absorption of helicid in rat everted intestinal sac model: A preliminary study

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