Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2022, Vol. 27 ›› Issue (9): 971-976.doi: 10.12092/j.issn.1009-2501.2022.09.002
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YANG Li1, BAI Huhu2, HE Shasha1, JIN Yue1, LIU Chengsong1
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Abstract: AIM: To investigate the relationship between TRPM3 and diabetes-induced painful peripheral neuropathy. METHODS: Treptozotocin (STZ) was intraperitoneal injected for establishment of diabetic mice model, behavioral tests of paw withdraw thresholds (PWTs) and paw withdraw latencies (PWLs) were conducted; Protein contents and tyrosine phosphorylation levels of TRPM3 were detected by immunoprecipitation and immunoblotting. RESULTS: The PWTs and PWLs in diabetic mice were significantly reduced; TRPM3 tyrosine phosphorylation in the dorsal root ganglia (DRG) of diabetic mice significantly increased compared with control, while the protein expression shows no statistical significance; Enhanced tyrosine phosphorylation of TRPM3 by BPV can evoke heat hyperalgesia in intact mice; Reduce of the tyrosine phosphorylation levels of TRPM3 through PP2 significantly alleviates diabetes-induced heat hyperalgesia, without affecting mechanical allodynia. CONCLUSION: The upregulation of tyrosine phosphorylation of TRPM3 plays a key role in heat related painful diabetic peripheral neuropathy.
Key words: TRPM3, DRG, diabetic peripheral neuropathy, tyrosine phosphorylation, hyperalgesia, allodynia
CLC Number:
R318.04
R338.3
R363.1
R966
YANG Li, BAI Huhu, HE Shasha, JIN Yue, LIU Chengsong. Tyrosine phosphorylation of TRPM3 ion channel mediates diabetes-induced heat hyperalgesia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(9): 971-976.
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URL: https://manu41.magtech.com.cn/Jweb_clyl/EN/10.12092/j.issn.1009-2501.2022.09.002
https://manu41.magtech.com.cn/Jweb_clyl/EN/Y2022/V27/I9/971