Construction strategy and application of an in vitro model of radioactive cardiac injury

doi:10.12092/j.issn.1009-2501.2026.05.008

Abstract

Abstract:

Radiation-induced heart disease is the most common and severe complication of radiation therapy for thoracic tumours. After exposure to radiation, cardiac tissue undergoes myocardial inflammation, fibrosis, and vascular endothelial damage, leading to cardiac dysfunction. With the continuous improvement of medical technology, the survival period of tumour patients has been significantly extended. However, RIHD severely impacts patients' quality of life. To thoroughly investigate its pathological mechanisms, establishing a reliable RIHD cell model is a crucial experimental foundation for studying its molecular mechanisms and evaluating treatment efficacy. This article reviews and summarises the application and research progress of RIHD cell models in related studies both domestically and internationally in recent years, aiming to provide a reliable theoretical basis for RIHD research.

Key words: radiation-induced, cellular model, standardization, drug screening

CLC Number:

R541
R730.55

Xiaying WANG, Hugang JIANG, Chunzhen REN, Jing MA, Jiakun LIU, Kai LIU, Yingdong LI, Xinke ZHAO. Construction strategy and application of an in vitro model of radioactive cardiac injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2026, 31(5): 639-648.

Figures/Tables 6

References 66

1	Chen J, Li M, Yu Y, et al. Prevention of ventricular arrhythmia complicating acute myocardial infarction by local cardiac denervation[J]. Int J Cardiol, 2015, 184, 667- 673. doi: 10.1016/j.ijcard.2015.03.057
2	Darby SC, Cutter DJ, Boerma M, et al. Radiation-related heart disease: current knowledge and future prospects[J]. Int J Radiat Oncol Biol Phys, 2010, 76 (3): 656- 665. doi: 10.1016/j.ijrobp.2009.09.064
3	Pan L, Lei D, Wang W, et al. Heart dose linked with cardiac events and overall survival in lung cancer radiotherapy: A meta-analysis[J]. Medicine (Baltimore), 2020, 99 (38): e21964. doi: 10.1097/md.0000000000021964
4	胡舜英, 邱乐, 段海峰, 等. 肝细胞生长因子在大鼠心肌细胞放射损伤中的抗凋亡作用[J]. 解放军医学杂志, 2007, 32 (5): 482- 484. doi: 10.3321/j.issn:0577-7402.2007.05.023
5	蒋虎刚. 基于益气活血理论探讨当归红芪多糖干预放射性心肌损伤的作用及分子机制 [D]. 兰州: 兰州大学, 2019.
6	邢喜平, 任春贞, 蒋虎刚, 等. 基于Rho/Rock通路探讨当归红芪多糖对辐射诱导大鼠H9C2心肌细胞的作用机制[J]. 辽宁中医杂志, 2021, 48 (7): 228- 231,262. doi: 10.13192/j.issn.1000-1719.2021.07.061
7	顾静. X射线诱导心肌成纤维细胞促纤维化损伤及黄芪注射液对其干预的实验研究 [D]. 西安: 陕西中医药大学, 2014.
8	郭欢. miR-21 对辐射后大鼠心脏成纤维细胞基因表达谱的影响及miR-21/GDF15促放射性纤维化机制研究 [D]. 兰州: 兰州大学, 2021.
9	Zeng ZM, Du HY, Xiong L, et al. BRCA1 protects cardiac microvascular endothelial cells against irradiation by regulating p21-mediated cell cycle arrest[J]. Life Sci, 2020, 244, 117342. doi: 10.1016/j.lfs.2020.117342
10	Yi J, Yue L, Zhang Y, et al. PTPMT1 protects cardiomyocytes from necroptosis induced by γ-ray irradiation through alleviating mitochondria injury[J]. Am J Physiol Cell Physiol, 2023, 324 (6): C1320- C1331. doi: 10.1152/ajpcell.00466.2022
11	Zeng Z, Xu P, He Y, et al. Acetylation of atp5f1c mediates cardiomyocyte senescence via metabolic dysfunction in radiation-induced heart damage[J]. Oxid Med Cell Longev, 2022, 2022, 4155565. doi: 10.1155/2022/4155565
12	刘侃玲. 自噬在PM2.5致冠状动脉微血管内皮细胞损伤中的作用及其机制 [D]. 广州: 南方医科大学, 2016.
13	徐祎琳. 白藜芦醇通过上调SIRT1表达介导对放射性心肌损伤的保护作用 [D]. 兰州: 兰州大学, 2022.
14	赵信科. 基于TNF-α/CTRP9/p42/44MAPK信号通路探讨当归红芪超滤物干预重离子束致心肌细胞损伤的氧化应激机制 [M]. 北京: 人民卫生出版社, 2022.
15	Cui WW, Ye C, Wang KX, et al. Momordica charantia-derived extracellular vesicles-like nanovesicles protect cardiomyocytes against radiation injury via attenuating DNA damage and mitochondria dysfunction[J]. Front Cardiovasc Med, 2022, 9, 864188. doi: 10.3389/fcvm.2022.864188
16	张文静. 丹参酮ⅡA磺酸钠对放射性心肌细胞损伤的保护作用及机制研究 [D]. 合肥: 安徽医科大学, 2016.
17	Edwards S, Adams J, Tchernikov A, et al. Low-dose X-ray radiation induces an adaptive response: A potential countermeasure to galactic cosmic radiation exposure[J]. Exp Physiol, 2024, 109 (8): 1123- 1132.
18	Gu A, Jie Y, Sun L, et al. RhNRG-1β protects the myocardium against irradiation-induced damage via the ErbB2-ERK-SIRT1 signaling pathway[J]. PLoS One, 2015, 10 (9): e0137337. doi: 10.1371/journal.pone.0137337
19	Wang G, Ma L, Wang B, et al. Tanshinone IIA accomplished protection against radiation-induced cardiomyocyte injury by regulating the p38/p53 pathway[J]. Mediators Inflamm, 2022, 2022, 1478181. doi: 10.1155/2022/1478181
20	贾成文. 当归红芪超滤物对miRNA-21-5P靶向调控TGF-β1/Smad3信号通路介导放射性心肌纤维化的干预效应 [D]. 兰州: 甘肃中医药大学, 2023.
21	孔姗姗, 汪鸣, 蒋虎刚, 等. 当归红芪超滤物对放射性H9C2细胞损伤TNF-α/CTRP9信号通路相关因子表达的影响[J]. 中国中医药信息杂志, 2021, 28 (5): 50- 54.
22	Li YL, Wang G, Wang BW, et al. The potential treatment of N-acetylcysteine as an antioxidant in the radiation-induced heart disease[J]. Cardiovasc Diagn Ther, 2024, 14 (4): 509- 524. doi: 10.21037/cdt-24-19
23	Gáspár R, Diószegi P, Nógrádi-Halmi D, et al. The proteoglycans biglycan and decorin protect cardiac cells against irradiation-induced cell death by inhibiting apoptosis[J]. Cells, 2024, 13 (10): 1485.
24	Kiscsatári L, Varga Z, Schally AV, et al. Protection of neonatal rat cardiac myocytes against radiation-induced damage with agonists of growth hormone-releasing hormone[J]. Pharmacol Res, 2016, 111, 859- 866. doi: 10.1016/j.phrs.2016.07.036
25	Li L, Nie X, Zhang P, et al. Dexrazoxane ameliorates radiation-induced heart disease in a rat model[J]. Aging (Albany NY), 2021, 13 (3): 3699- 3711. doi: 10.18632/aging.202332
26	Chang J, Ma C, Guo H, et al. Ultrafiltration extract of radix angelica sinensis and radix hedysari attenuates risk of low-dose x-ray radiation-induced myocardial fibrosis in vitro[J]. Evid Based Complement Alternat Med, 2021, 2021, 5580828. doi: 10.1155/2021/5580828
27	Xu P, Yi Y, Xiong L, et al. Oncostatin m/oncostatin m receptor signal induces radiation-induced heart fibrosis by regulating SMAD4 in fibroblast[J]. Int J Radiat Oncol Biol Phys, 2024, 118 (1): 203- 217. doi: 10.1016/j.ijrobp.2023.07.033
28	Wang KX, Ye C, Yang X, et al. New insights into the understanding of mechanisms of radiation-induced heart disease[J]. Curr Treat Options Oncol, 2023, 24 (1): 12- 29. doi: 10.1007/s11864-022-01041-4
29	Wu B, Zhao S, Zhang J, et al. PD-1 inhibitor aggravate irradiation-induced myocardial fibrosis by regulating tgf-β1/Smads signaling pathway via GSDMD-mediated pyroptosis[J]. Inflammation, 2024, 47 (6): 2145- 2158.
30	Sun T, Liu H, Cheng Y, et al. 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-β-d-glucoside eliminates ischemia/reperfusion injury-induced H9c2 cardiomyocytes apoptosis involving in Bcl-2, Bax, caspase-3, and Akt activation[J]. J Cell Biochem, 2019, 120 (7): 10972- 10977.
31	Hu C, Zhang X, Wei W, et al. Matrine attenuates oxidative stress and cardiomyocyte apoptosis in doxorubicin-induced cardiotoxicity via maintaining AMPKα/UCP2 pathway[J]. Acta Pharm Sin B, 2019, 9 (4): 690- 701. doi: 10.1016/j.apsb.2019.03.003
32	Elrod JW, Wong R, Mishra S, et al. Cyclophilin D controls mitochondrial pore-dependent Ca(2+) exchange, metabolic flexibility, and propensity for heart failure in mice[J]. J Clin Invest, 2010, 120 (10): 3680- 3687. doi: 10.1172/JCI43171
33	Zamorano JL, Lancellotti P, Rodriguez Muñoz D, et al. 2016 ESC position paper on cancer treatments and cardiovascular toxicity developed under the auspices of the ESC committee for practice guidelines: the task force for cancer treatments and cardiovascular toxicity of the european society of cardiology (ESC)[J]. Eur Heart J, 2016, 37 (36): 2768- 2801. doi: 10.1093/eurheartj/ehw211
34	Moussad EE, Brigstock DR. Connective tissue growth factor: what's in a name?[J]. Mol Genet Metab, 2000, 71 (1-2): 276- 292. doi: 10.1006/mgme.2000.3059
35	Pan X, Chen Z, Huang R, et al. Transforming growth factor β1 induces the expression of collagen type I by DNA methylation in cardiac fibroblasts[J]. PLoS One, 2013, 8 (4): e60335. doi: 10.1371/journal.pone.0060335
36	Collins T, Cybulsky MI. NF-kappaB: pivotal mediator or innocent bystander in atherogenesis?[J]. J Clin Invest, 2001, 107 (3): 255- 264. doi: 10.1172/JCI10373
37	Lebranchu Y, Bridoux F, Büchler M, et al. Immunoprophylaxis with basiliximab compared with antithymocyte globulin in renal transplant patients receiving MMF-containing triple therapy[J]. Am J Transplant, 2002, 2 (1): 48- 56. doi: 10.1034/j.1600-6143.2002.020109.x
38	Caligiuri A, Gitto S, Lori G, et al. Oncostatin M: from intracellular signaling to therapeutic targets in liver cancer[J]. Cancers (Basel), 2022, 14 (17): 4162.
39	Nakajima H, Nakajima HO, Salcher O, et al. Atrial but not ventricular fibrosis in mice expressing a mutant transforming growth factor-beta (1) transgene in the heart[J]. Circ Res, 2000, 86 (5): 571- 579. doi: 10.1161/01.RES.86.5.571
40	Chia ZJ, Cao YN, Little PJ, et al. Transforming growth factor-β receptors: versatile mechanisms of ligand activation[J]. Acta Pharmacol Sin, 2024, 45 (7): 1337- 1348. doi: 10.1038/s41401-024-01235-6
41	Mathias D, Mitchel RE, Barclay M, et al. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice[J]. PLoS One, 2015, 10 (3): e0119661. doi: 10.1371/journal.pone.0119661
42	Baselet B, Sonveaux P, Baatout S. Pathological effects of ionizing radiation: endothelial activation and dysfunction[J]. Cell Mol Life Sci, 2019, 76 (4): 699- 728. doi: 10.1007/s00018-018-2956-z
43	Preidl RHM, Möbius P, Weber M. Correction to: Long-term endothelial dysfunction in irradiated vessels: an immunohistochemical analysis[J]. Strahlenther Onkol, 2020, 196 (5): 485. doi: 10.1007/s00066-019-01564-0
44	Patties I, Haagen J, Dörr W. Late inflammatory and thrombotic changes in irradiated hearts of C57BL/6 wild-type and atherosclerosis-prone ApoE-deficient mice[J]. Strahlenther Onkol, 2015, 191 (2): 172- 179. doi: 10.1007/s00066-014-0745-7
45	Halle M, Gabrielsen A, Paulsson-Berne G, et al. Sustained inflammation due to nuclear factor-kappa B activation in irradiated human arteries[J]. J Am Coll Cardiol, 2010, 55 (12): 1227- 1236. doi: 10.1016/j.jacc.2009.10.047
46	杜海洋. BRCA1/p21基因敲除对小鼠放射性心脏损伤的影响 [D]. 北京: 北京协和医学院, 2020.
47	Liu T, Zhang L, Joo D, et al. NF-κB signaling in inflammation[J]. Signal Transduct Target Ther, 2017, 2, 17023. doi: 10.1038/sigtrans.2017.23
48	闫冰川, 苏旭春, 孔嘉欣, 等. 参芪扶正注射液预防放射性心脏损伤临床观察[J]. 湖南中医杂志, 2012, 28 (4): 4- 6.
49	钱永红. 生脉注射液防治放射性心脏损伤疗效观察[J]. 北方药学, 2014, 11 (7): 51- 52.
50	Ziegler V, Henninger C, Simiantonakis I, et al. Rho inhibition by lovastatin affects apoptosis and DSB repair of primary human lung cells in vitro and lung tissue in vivo following fractionated irradiation[J]. Cell Death Dis, 2017, 8 (8): e2978. doi: 10.1038/cddis.2017.372
51	Talebpour Amiri F, Hamzeh M, Naeimi RA. Radioprotective effect of atorvastatin against ionizing radiation-induced nephrotoxicity in mice[J]. Int J Radiat Biol, 2018, 94 (2): 106- 113. doi: 10.1080/09553002.2018.1420926
52	Kang J, Kim YC, Park JJ, et al. Increased epicardial adipose tissue thickness is a predictor of new-onset diabetes mellitus in patients with coronary artery disease treated with high-intensity statins[J]. Cardiovasc Diabetol, 2018, 17 (1): 10. doi: 10.1186/s12933-017-0650-3
53	Krum H, Schlaich MP, Sobotka PA, et al. Percutaneous renal denervation in patients with treatment-resistant hypertension: final 3-year report of the Symplicity HTN-1 study[J]. Lancet, 2014, 383 (9917): 622- 629. doi: 10.1016/S0140-6736(13)62192-3
54	Chaldakov GN. Colchicine, a microtubule-disassembling drug, in the therapy of cardiovascular diseases[J]. Cell Biol Int, 2018, 42 (8): 1079- 1084. doi: 10.1002/cbin.10988
55	李鹏. RIHD小鼠模型的建立与评价及天麻素对其影响的研究 [D]. 遵义: 遵义医科大学, 2020.
56	沈伟生, 夏德洪, 高春恒, 等. 参松养心胶囊加红景天干预放射性心脏损伤的临床研究[J]. 南京中医药大学学报, 2016, 32 (5): 431- 434.
57	杨旭光, 田迎霞, 沈蓉, 等. 放射性心脏损伤的机制及治疗研究进展[J]. 兰州大学学报(医学版), 2022, 48 (2): 81- 86.
58	冯璟, 于远望. 当归补血汤对辐射损伤小鼠的防护作用[J]. 中国比较医学杂志, 2015, 25 (10): 55- 58.
59	范习刚, 吴飞, 杨桄权, 等. 黄芪生脉饮联合阿司匹林对胸部肿瘤放疗后放射性心脏损伤的防治作用研究[J]. 世界中西医结合杂志, 2022, 17 (1): 111- 115.
60	Li H, Cao L, Yi PQ, et al. Pituitary adenylate cyclase-activating polypeptide ameliorates radiation-induced cardiac injury[J]. Am J Transl Res, 2019, 11 (10): 6585- 6599. doi: 10.1016/j.ijrobp.2019.06.1051
61	畅艳娜, 李应东, 王雪梅, 等. 当归红芪超滤物抗辐射致心肌细胞损伤的作用及机制[J]. 世界中西医结合杂志, 2014, 9 (5): 487- 491.
62	徐梦游. 参麦注射液在放射性心脏损伤中的保护作用研究 [D]. 南京: 南京中医药大学, 2021.
63	Ping Z, Peng Y, Lang H, et al. Oxidative stress in radiation-induced cardiotoxicity[J]. Oxid Med Cell Longev, 2020, 2020, 3579143.
64	顾静. 中医药对放射性心脏纤维化损伤的尝试性干预研究 [C]. 中国生理学会张锡钧基金第十五届全国青年优秀生理学学术论文交流及评奖会议; 中国生理学会第十三届全国青年生理学工作者学术会议, 2019: 2.
65	辛二旦, 张育贵, 边甜甜, 等. 黄芪甲苷对心血管疾病的作用机制研究状况[J]. 中国临床药理学杂志, 2024, 40 (17): 2580- 2585. doi: 10.13699/j.cnki.1001-6821.2024.17.026
66	Wu X, Zhi X, Liu K, et al. Prevention and control of cardiac arrhythmic by using therapeutic foods: a review[J]. J Cardiovasc Electrophysiol, 2024, 35 (11): 1892- 1901. doi: 10.1111/jce.16428

Detection target	Molecular biomarkers/functional indicators	Detection techniques	References
Oxidative stress	ROS levels, SOD activity, MDA content	DCFH-DA fluorescent probe	[6]
Apoptosis/vecrosis	Caspase-3 activity, Bax/Bcl-2 ratio, LDH release	Annexin V/PI double staining, Western Blot, LDH assay kit	[7]
Cell viability and proliferation	Cell survival rate, proliferation curve staining	CCK-8/MTT assay, EdU staining	[8]
DNA damage	γ-H2AX foci, ATM/ATR phosphorylation	Immunofluorescence, Western Blot	[9]
Fibrosis	TGF-β1, α-SMA, CollagenⅠ/Ⅲ	ELISA, Immunofluorescence, Sirius red staining	[10]
Mitochondrial dysfunction	ATP content, membrane potential (ΔΨm), mtROS	JC-1 probe (Flow cytometry), Seahorse XF Analyzer (oxygen consumption rate)	[24]
Inflammatory response	IL-6, TNF-α, IL-1β	(Luminex), RT-qPCR Multiplex liquid chip (Luminex), RT-qPCR (mRNA level)	[28]

Detection target	Molecular biomarkers/functional indicators	Detection techniques	References
Oxidative stress	ROS levels, SOD activity, MDA content	DCFH-DA fluorescent probe	[6]
Apoptosis/vecrosis	Caspase-3 activity, Bax/Bcl-2 ratio, LDH release	Annexin V/PI double staining, Western Blot, LDH assay kit	[7]
Cell viability and proliferation	Cell survival rate, proliferation curve staining	CCK-8/MTT assay, EdU staining	[8]
DNA damage	γ-H2AX foci, ATM/ATR phosphorylation	Immunofluorescence, Western Blot	[9]
Fibrosis	TGF-β1, α-SMA, CollagenⅠ/Ⅲ	ELISA, Immunofluorescence, Sirius red staining	[10]
Mitochondrial dysfunction	ATP content, membrane potential (ΔΨm), mtROS	JC-1 probe (Flow cytometry), Seahorse XF Analyzer (oxygen consumption rate)	[24]
Inflammatory response	IL-6, TNF-α, IL-1β	(Luminex), RT-qPCR Multiplex liquid chip (Luminex), RT-qPCR (mRNA level)	[28]

Drugs	Cell model	Dosage	Time	Method	Mechanisms	Therapeutic effects	References
Angelica-hedysarum polysaccharide	H9C2 irradiated with 2 Gy X-ray, detected at 24 h	400 mg/L	24 h	DEME	Rho/Rock↓	Anti-fibrotic, inhibits myocardial remodeling	[51]
Astragalus injection	CFs irradiated with 2 Gy X-ray, detected at 48 h	10 μg/mL 20 μg/mL	48 h	DEME	TGF-β1↓ Col-Ⅰ↓	Reduces ROS, inhibits ER stress and fibrosis	[7]
Shenmai injection	H9C2 irradiated with 20 Gy X-ray, detected at 72 h	1 μL/mL 5 μL/mL	72 h	DEME	ROS↓	Inhibits myocardial fibrosis and macrophage infiltration	[62]
Salidroside	H9C2 irradiated with 6 Gy X-ray, detected at 48 h	0.50 mmol/L	24 h	DEME	Bcl-2/Bax↑	Anti-inflammatory; mitigates cardiac injury	[50]
Angelica- hedysarum ultrafiltrate	H9C2 irradiated with 6 Gy X-ray, detected at 24 h	3,6,9 mg/mL	48 h	DEME	LDH↓, Bax, SOD↑	Antioxidant; reduces myocardial cell damage	[61]
Resveratrol	H9C2 irradiated with 4 Gy X-ray, detected at 48 h	50 μmol/L	24 h	DEME	TGF-β1↓	Alleviates oxidative stress and inflammation	[63]
Astragalus polysaccharide	H9C2 irradiated with 4 Gy X-ray, detected at 24 h	400 μg/mL	48 h	DEME	PI3K/Akt/ mTOR↑	Improves radiation-induced autophagic flux; reduces apoptosis and atrophy	[52]
Astragalosides	CFs irradiated with 1 Gy X-ray, detected at 48 h	20 mg/mL	48 h	DEME	TGF-β1 Col-Ⅰ↓	Reduces ROS; alleviates radiation-induced fibrosis	[64]
Astragaloside IV	HUVECs irradiated with 4 Gy X-ray, detected at 24 h	100 μg/mL	12 h	DEME	SOD↑	Anti-inflammatory; mitigates cardiac injury	[65]

Drugs	Cell model	Dosage	Time	Method	Mechanisms	Therapeutic effects	References
Angelica-hedysarum polysaccharide	H9C2 irradiated with 2 Gy X-ray, detected at 24 h	400 mg/L	24 h	DEME	Rho/Rock↓	Anti-fibrotic, inhibits myocardial remodeling	[51]
Astragalus injection	CFs irradiated with 2 Gy X-ray, detected at 48 h	10 μg/mL 20 μg/mL	48 h	DEME	TGF-β1↓ Col-Ⅰ↓	Reduces ROS, inhibits ER stress and fibrosis	[7]
Shenmai injection	H9C2 irradiated with 20 Gy X-ray, detected at 72 h	1 μL/mL 5 μL/mL	72 h	DEME	ROS↓	Inhibits myocardial fibrosis and macrophage infiltration	[62]
Salidroside	H9C2 irradiated with 6 Gy X-ray, detected at 48 h	0.50 mmol/L	24 h	DEME	Bcl-2/Bax↑	Anti-inflammatory; mitigates cardiac injury	[50]
Angelica- hedysarum ultrafiltrate	H9C2 irradiated with 6 Gy X-ray, detected at 24 h	3,6,9 mg/mL	48 h	DEME	LDH↓, Bax, SOD↑	Antioxidant; reduces myocardial cell damage	[61]
Resveratrol	H9C2 irradiated with 4 Gy X-ray, detected at 48 h	50 μmol/L	24 h	DEME	TGF-β1↓	Alleviates oxidative stress and inflammation	[63]
Astragalus polysaccharide	H9C2 irradiated with 4 Gy X-ray, detected at 24 h	400 μg/mL	48 h	DEME	PI3K/Akt/ mTOR↑	Improves radiation-induced autophagic flux; reduces apoptosis and atrophy	[52]
Astragalosides	CFs irradiated with 1 Gy X-ray, detected at 48 h	20 mg/mL	48 h	DEME	TGF-β1 Col-Ⅰ↓	Reduces ROS; alleviates radiation-induced fibrosis	[64]
Astragaloside IV	HUVECs irradiated with 4 Gy X-ray, detected at 24 h	100 μg/mL	12 h	DEME	SOD↑	Anti-inflammatory; mitigates cardiac injury	[65]

[1]	Xiaying WANG, Hugang JIANG, Jiakun LIU, Jing MA, Kai LIU, Yingdong LI, Xinke ZHAO. Effect of Angelica astragalus ultrafiltration on cardiomyocytes induced by ionizing radiation and its mechanism [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2026, 31(3): 300-312.
[2]	CAO Zhiwen, YAN Guijun. Research progress of uterine endometrial epithelial cell organoids in the field of reproduction [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2024, 29(5): 576-582.
[3]	HE Liyang, WEI Wenjie, YIN Qin, LIU Lijing, ZHONG Boying, LIU Cungen, ZHOU Meiling, HE Jianbin. Preventive and therapeutic effects of the Kiwi fruit essence (unsaturated fatty acid of actinidia chinesis planch seed oil) on radiation-induced lung injury rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(2): 154-162.
[4]	JIA Chengwen, JIANG Hugang, GAO Xiang, HAN Jinyan, XU Xiaodong, ZHAO Xinke. Interventional effect of Radix Angelica Sinensis and Radix Hedysari ultrafiltration on miR-21-5P targeting TGF-β1-mediated radiation-induced myocardial fibrosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1221-1230.
[5]	ZHAO Yin, LI Qin, ZHANG Xin-yue. Study progress of type I anaphylaxis reaction and anaphylactoid reaction on the basis of RBL-2H3 cell models [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(11): 1310-1314.
[6]	Li Ting, ZHANG Xiao-dong, SONG Yu-wen, LIU Jian-wen. A microplate-based screening method for alpha-glucosidase inhibitors [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(10): 1128-1134.
[7]	CHENG Yu-fang, XU Jiang-ping. Cell adhesion molecules and new drug research [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(1): 5-8.