Nerve growth factor prevents nitric oxide release and nitric oxide synthase gene expression induced by glutamate in primary cortical cultures of fetal rats

Abstract

Abstract: Aim To study the effects of nerve growth factor (NGF) on nitric oxide (NO ) release and constitutive nitricoxide synthase (cNOS) geneexpression induced by glutam ate(Glu) in cortical neuronal cuItures.Methods Neuron viability was measured to assay NGF effect.NO content and the expression of cNOS mRNA w ere determined by fluo-rometric method and Northe rn blotting respectively.Results Glu (0.5~2.0 mmolL)induced a marked increase of neuronal death and NO content in cultured cerebral cortical neurons.L-NAME (100μmol/L) and NGF (100μg/L) signifi cantly inereased neuronal surviving and decreased NO release.Hemolglobin (Hb),which binds NO,completely prevented Gluinduced cell death and NO release at 500 pμmoY L.NGF (50,100μg/L)sigmificantly inhibited cNOS m RNA expression induced by Glu (0.5 mmoYL).Conclusion NO mediates Gluneurotoxicity in cortical neuronal cultures and NGF protects the neurons against Gluneurotoxicity viainhibiting the activity of cNOS and the release of NO.

Key words: nerve growth factor, cerebral cortex, cell, culture, glutamie acid, nitric oxide, nitric oxide synthase

CLC Number:

R969.2

WU Jun-Fang, WANG Ping, ZHANG Jun-Tian. Nerve growth factor prevents nitric oxide release and nitric oxide synthase gene expression induced by glutamate in primary cortical cultures of fetal rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 1998, 3(4): 241-244.

References

1 Moncada S,Palmer RMJ,Hggs EA.Nitric oxide phy siology,pathophysiology and pharma-cology.Pharmacol Rev,1991:43:109
2 Schmidt HHHW,Walter U.No at work.Cell,1994;78:919
3 Li P,Tong CY,Eisenach JC.NMDA causes re-lease of nitrie oxide from rat spinal cord in vitro.Brain Res,1994;637:287
4 Dawson VL,Dawson TM,London ED,et al.Nitrie oxide mediates glutamate neurotoxicity in.primary cortical cultures.Proe Natl Acad Sa USA,1991;88:6368
5 Mattson MP,Lovell M A,Furukawa K,et al.N eurotrophie factors at tenuate glutamate -induced accumulation of pe roxides,elev ation of int racellu-lar Cag²⁺concent ration,and neurotoxicity and in-cre ase antioxidant enzyme activities in hippocam-pal neurons.J Neurochem,1995;65:1740
6 ShimohamaS,Ogawa N,Tamura Y,et al.Pro-tective efct of nerve growth factor against gluta-ma te induced neurotoxicity in cultured cortical.neurons.Brain Res,1993;632:296
7 Hong QT,Tang YP.Neurons of cerebral ortex of new born rats in primary culture.Chin J Neu-roanat,1994:10:259
8 Cheng B,Mattson MP.NT-3 and BDNF protect hippocampal,septal,and cortical neurons against met abolie compromise.Brain Resr1994640:56
9 Wei G,Zhang JT.The content of NO was mea-sured by spectrofluorometrice method in brain of rats.Chin Pharmacol Bulletin,1996:12:470
10 Gough NM.Rapid and quantitative preparation of cytoplasmic RNA from sm all mumbers of cells.Anal Biochem,1988;173:93
11 Sambrook J,Fritsch EF,Maniatis Teds.M olee-ular Cloning A Laboratory Manual.New York:CSH.1989:739
12 Bredt DS,Snyder SH.Isolation of nit rie ox ide synthase,a calm odulinrequiring enzyme.Proe Natl Acad Sci USA,1990;87:682.
13 Li JQ,Li ZK,Duan H,et al.Effeet of age and ginseroside Rgl on nitrie oxide content and nitrie oxide synthase activity of cerebral cortex in rats.Acta P harm aceu Sin,1997;32:251
14 Lin L,Zhang HY,Gu HM,et al.Nerve growth factors prevent glutamate toxiaity in cortical neu-ronal cultures.Acta Pharmacol,Sin,1996;17:221

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