Expression ofμandκopiate receptor in spinal cord and brainstem during morphine withdrawal in rats

Abstract

Abstract: Aim To observe the gene expression ofμandκopiate receptor in spinal cord and brainstem,and the effects of muscarinic receptor antagonist,NMDA receptor antagonists and inhibitor of nitricoxide synthase on the expression of these genes during morphine withdrawal in rats.Methods The mRNA levels ofμandκopiate receptor mRNA were assayed by reverse transcription polymerase chain reaction (RT-PCR)with the beta-actin mRNA as an internal control.Results Theμopiate receptor mRNA levels were increased significantly in spinal cord and brainstem during morphine dependence,and decreased after injection of naloxone during morphine withdrawal in rats.Theκopiate receptor mRNA levels in spinal cord and brainstem were changed conversely compared with theμopiate receptor mRNA levels during morphine dependence and withdrawal.Theμandκopiate receptor mRNA levels in spinal cord and brainstem were decreased by administration of either Rp-cAMPs or calyculin A while these levels were not changed by Sp-cAMPs at half hour before injection of naloxone in morphine dependent rats.Administration of l-N-nitric arginine methylester(10mg·kg^-1)resulted in a decrease ofμopiate receptor andκopiate receptor levels in spinal cord,andμopiate receptor levels in spinal cord andκopiate receptor levels in brainstem were dedcreased by pretreatment with methyl-scopolamine (0.5mg·kg^-1)during morphine withdrawal.However,theμandκopiate receptor levels in both spinal cord and brainstem were not different from those of morphine withdrawal rats pretreated with either MK801 (0.125mg·kg^-1)or pirezenpine(10mg·kg^-1).In adddition,β-actin mRNA levels were not different in each group.Conclusion The expression ofμopiate receptor andκopiate receptor mRNA plays an important role in mediating the process of morphine dependence and withdrawal,and the expression ofμopiate receptor andκopiate receptor mRNA in spinal cord and brainstem could be inhibited by block of muscarinic receptor or inhibition of nitric oxide production.

Key words: morphine, withdrawal, μ opiate receptor, κ opiate receptor, muscarinic receptor, NMDA receptor

CLC Number:

R971.1

LIU Hui-Fen, XIE Xiao-Hu, ZHOU Wen-Hua, YANG Guo-Dong. Expression ofμandκopiate receptor in spinal cord and brainstem during morphine withdrawal in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2000, 5(1): 1-5.

References

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