Abstract: Aim To observe homoharring to nine(HHT)distribution in eyeball tissue of rabbit.Methods Harringtonine(HT)was used as internal standard.The chromatography consisted of Kromasil C₁₈ column with methanol0.01mol·L^-1(60∶40,pH_2.5)as the mobile phase at the flow rate of 0.8ml·min^-1.Results The chromatography retention time of HHT and HT were6.35min and 4.70min.The standard curve was linear in the concentration range of 5~200μg·L^-1in serum and the relation coefficient was 0.994.The recovery of spiked sample was(93.17±4.31)%.After injection of 2mg of HHT in side of rabbit eyeball,the contents in sclera,cornea,retina,vitreous body and crystalline lens were reduced progressively,but the eliminate rate w ere reduced in a proper order.Conclusion HHT can distribute to every eyeball tissue when it was injected in side of rabbit eyeball.

Key words: homoharringtonine, distribution, high performance liquid chromatography