Lack effect of metabotropic glutamate receptors on neurotoxicity of PC12 cells induced by 6-OHDA1

Abstract

Abstract: AIM: To explore if metabotropic glutamate receptors (mGluRs) induces neuroprotection against 6-hydroxydopamine (6-OHDA) neurotoxicity in PC12 cells. METHODS: The alteration of the glutamate in extracellular fluid of PC12 cells was detected by high performance liquid chromatography (HPLC) with a fluorescent detector.The cytotoxic activity of PC12 cells was assayed by means of MTT colorimetric method. RESULTS: 6- OHDA dose-dependently increased glutamate release and decreased cell activity of PC12 cells, and the mGluRs ligands had no significant effect on that. CONCLUSION: The mGluR3 ligands has no protective effect on neurotoxicity of PC12 cells induced by 6-OHDA.

Key words: pharmacology, metabotropic glutamate receptors, PC12 cells, 6-hydroxydopamine, glutamate, cell viability

CLC Number:

MENG Chang-Hong, DING Jian-Hua, HU Gang. Lack effect of metabotropic glutamate receptors on neurotoxicity of PC12 cells induced by 6-OHDA1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(3): 269-272.

References

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