Abstract: AIM: To approach the immunoregulation function and mechanism of compound zhongjiling. METHODS: EAMG models in Lews rats were established.By using 3H-TdR method, the effect of zhongjiling to proliferation reaction of lymphocyte in normal BALB/C spleen of mice induced by ConA and Lps and the effect of compound zhongjiling to proliferation reaction of lymphocyte in lymph node of rats was examined;by using ELISA, the effect of zhongjiling to AchRab titre and the level of IFN-γin serum of EAMG rats was examined. RESULTS: there was no significant improvement on the effect of zhongjiling to proliferation reaction of lymphocyte in normal BALB C spleen of mice induced by ConA and LPS(P <0.05).The effect of compound zhongjiling on the proliferation reaction of lymphocyte showed a significant improvement (P <0.01) in lymph node of EAMG rats induced by ConA, and the inhibition was induced by N₂AchR (P <0.01).The AchRab titre and the level of IFN-γ significantly reduced (P <0.01) in serum of EAMG rats. CONCLUSIONs: The mechanism of compound zhongjiling is not through immunosuppression, but through restraining N₂AchR antigen to induce specific immune reaction in prevention and treatment of EAMG.

Key words: pharmacology, compound zhongjiling, EAMG, immunoregulation, IFN-γ