Abstract: AIM: To study the effects ofl-menthol and its binary systems onPercutaneousPenetration and deposit effects of 5-fluorouracil(5-FU).METHODS: Percutaneous absorption experiment wasPerformed on the apparatus of isolated skin,penetrated amounts of different times of 5-FU were determined,the cumulatePenetrated amounts andPermeation rates and increasedPermeation times were calculated,and the deposit effect of 5-FU was investigated.RESULTS: L-menthol of different concentrations could significantly enhance thePercutaneousPenetration of 5-FU,its increasedPermeation times were1.25 and 1.45 and 1.37 times respectively,and the action of 5-FU with 2 %l-menthol was the strongest.5-FU with 2 % and 4 %l-menthol had a marked deposit effect.When some kinds of enhancers were used alone or combined withl-menthol,thePercutaneousPenetration of 5-FU had a significantly increase.CONCLUSION: Lmenthol and its binary systems can markedlyPromote thePercutaneousPenetration and the deposit effect of 5-FU.

Key words: pharmacology, l-menthol, binary systems, 5-fluorouracil, permeation rates, increasedPermeation times, deposit effect