Regulatory effects of intracellular calcium in cultured endothelial cells through activating endothelial target for acetylcholine

Abstract

Abstract: AIM: To examine intracellular calciumlevels in bovine aortic endothelial cells(BAEC)and human coronary aortic endothelial cells(HCAEC)by activating the endothelial target for acetylcholine(ETA).METHODS: As fluorescenceProbe,Fluo-3 was used in assaying [Ca²⁺]i in endothelial cells by cofocus.Nonselective agonists of cholinery receptors acetylcholine and carbachol were tested.RESULTS: Acetylcholine and carbachol can elicit the [Ca²⁺]i elevation between the 3rd to 10thPassage of BAEC or 8thPassage of HCAEC at the concentration between 10^-5and 10^-2mol·L^-1.CONCLUSION: ETA can be activated by acetylcholine and carbachol and mediated the rise of intracellular Ca^{2 +}in BAEC and HCAEC until the 10thPassage.This characteristic of ETA is notlost in culturedPassages.

Key words: pharmacology, endothelial cell, the endothelial target for acetylcholine, [Ca²⁺] i, cofocus

CLC Number:

R965
R971.91

CHEN Dong-Mei, YU Min, MU Shao-Feng, WANG Hai. Regulatory effects of intracellular calcium in cultured endothelial cells through activating endothelial target for acetylcholine[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(6): 608-612.

References

1 Furchgott RF,Zawadzki JV.The obligatory role of endothelial cells in the relaxation of arterialsmooth muscle by acetylcholine[J].Nature,1980;288:227-3
2 Cocks TM,Angus JA,Campbell JH.Release andProperties of endothelium-derived relaxing factor(EDRF)from endothelial cells in culture[J].J CellPhysiol,1985;123:310-20
3 Huang TY,Chu TF,Chen HI.Heterogeneity of [Ca²⁺] i signaling in intact rat aortic endothelium[J].FASEB J,2000;14:797-804
4 Miwa Y,Hirata K,Kawashima S.Lysophosphatidylcholine inhibits receptor-mediated Ca²⁺ mobilization in intact endothelial cells of rabbit aorta[J].Arterioscler Thromb Vasc Biol,1997;17:1561-7
5 Wang XD,Lau F,Lil.Acetylcholine-sensitive intracellular Ca²⁺ store in fresh endothelial cells and evidence for ryanodine receptors[J].Circ Res,2003;77:37-42
6 Tracey WR,Peach MJ.Differential muscarinic receptor mRNA expression by freshly isolated and cultured bovine aortic endothelial cells[J].Circ Res,1992;70:234-40
7 Busse R,Fichtner H,Luckhoff A.Hyperpolarization and increased free calcium in acetylcholine-stimulated endothelial cells[J].Am JPhysiol,1988;255:H965-H969
8 Mehrke G,Poho U,Daul J.Effects of vasoactive agonists on the membranePotential of cultured bovine aortic and guinea-pig coronary[J].JPhysiol(Lond),1991;439:277-99
9 lynchM,Gillespie JI,Greenwell JR.Intracellular calcium signatures evoked by different agonists in isolated bovine aortic endothelial cells[J].Cell Calcium,1992;13:227-33
10 Gordon JL,Martin W.Endothelium-dependent relaxation of thePig aorta:relationshiPto stimulation of 86Rb efflux from isolated endothelial cells[J].Br JPharmacol,1983;79:531-41
11 Olesen SP,DaviesPF,Clapham DE.Muscarinic-activated K+ current in bovine aortic endothelial cells[J].Circ Res,1988;62:1059-64
12 Archer SL,Cowan NJ.Measurement of endotjelial cytosolic calcium concentration and nitric oxideProduction reveals discrete mechanisms of endothelium-dependentPulmonary vasodilation[J].Circ Res,1991;68:1569-81
13 ShanlM,Wang H.Pharmacological characteristics of the endothelial target for acetylcholine[J].Life Sci,2002;70:1285-98
14 陈冬梅,慕邵峰,汪海.激活血管内皮细胞乙酰胆碱作用靶标的抗血栓作用及其分子机制[J].中国药理学通报,2002;18:527-31
15 ClozelM.Pathophysiological role of endothelin revealed by the first orally active endothelin receptor antagonist[J].Nature,1994;365:759-63

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