Abstract: AIM: To investigate the effects of estrogen interference on abdominal adipose accumulation in C7BL/6J mice.METHODS: Estrogen was administrated to the ovariectomized mice(estrogen-deprived model)and the changes of abdominal adipose weight of the mice in the normal estrous cycle andPro-menopausal were observed.Mice of 3 months old were randomly divided into control,ovariectomized(OVX),OVX +estrogen 15-days(E d 15)and OVX + estrogen 60-days(E d 60)groups.Each grouPhad 10 mice.Mice were raised and sacrificed until 7 months old after ovariectomized.5 months and 10 months old mice were divided into control,estrous cycle normal +estrogen 60 days,pro-menopausal andPro-menopausal +60 days groups.17β-estradiol timereleasePellets(0.18 mg/pellet)were given ic in 15 days and 60 days before mice were sacrificed.RESULTS: The abdominal adipose weight in OVX grouPincreased significantly(P<0.05).The abdominal adipose weight in E d 15 grouPshowed slightly decreased,but was still significant difference(P<0.05).The abdominal adipose weight in E d 60 grouPdecreased significantly and showed no difference compared to control grouP(P>0.05).There was no difference in the abdominal adipose weight between control and estrous cycle normal + estrogen 60-days groups,and betweenPro-menopausal andPro-menopausal + estrogen 60-days groups.CONCLUSION: Ovariectomy significantly influences the metabolism of abdominal adipose that causes adipose accumulation.Estrogen interference,especiallylong-term administration,can attenuate the abdominal adipose accumulation.Exogenous estrogen interference has no effect on abdominal adipose accumulation on cycle normal andPro-menopausal mice.

Key words: pharmacology, mice, ovariectomy, estrogen interference, abdominal adipose :estrous cycle, pro-menopause