Abstract: AIM: To observe theProtective effects of shenmai injection on myocardial injury induced by 1,1-dipheyl-2-picryl hydrazyl(DPPH)free radical in isolated rabbit hearts.METHODS: The isolated rabbit hearts werePerfused in alangendorff model.Left ventricularPressure(lVP),the first derivative oflVP(+dp/d tmax),left ventricular end-diastolicPressure(lVEDP)and heart rate(HR)were recorded.Coronary flow(CF)and the activity of creatine kinase(CK)in coronary effluent were measured by timed collection of coronary effluent.RESULTS:Perfusion with DPPH(0.25μmol·L^-1)for 10 min caused a significant impairment of cardiac function,as shown by a decrease inlVP,+dp/dtmax and HR and an increase inlVEDPas well as the increased release of CK.AfterPerfusion with DPPH(0.25μmol·L^-1)for 10min,treatment with shenmai injection(1∶320 or 1∶160)for 10minProduced a significant improvement of cardiac function and a decrease in the release of CK.CONCLUSION: Shenmai injectionProtects against myocardial injury induced by DDPH free radical in isolated rabbit hearts.

Key words: pharmacology, shenmai injection, DPPH, free radical, heart, rabbit