Observation of morphology in Escherichia coli with atom microscope during the cefmetazole-induced post-antibiotic effect period

Abstract

Abstract: AIM: To study the mechanism of postantibiotic effect (PEA) by observing the changes of morphology.METHODS: The morphological effects of Escherichia coli were studied with atomic force microscopy (AFM) during the cefmetazole-induced post-antibiotic effect.RESULTS: The cefmetazole-induced (4 ×MIC, 128 ×MIC) PAE as defined by viability counting were-0. 03 h and 3. 65 h in Escherichia coli There was noted formation of filamentous structures at low concentrations (4 ×MIC) and the formations of spheroplasts at higher concentrations (128 ×MIC) by atomic force microscopy (AFM), and this morphological form persisted past the classically defined PAE.CONCLUSION: The mechanism of PAE is different at different concentrations through the inhibition of different protective bacterial enzymes.

Key words: cefmetazole, post-antibiotic effect, Escherichia coli, atomic force microscopy, morphology

CLC Number:

R978.11

ZHANG Yong-Qing, WANG Rui, ZHU Man, ZHANG Jian-Peng, ZHANG Yong-Mei, TONG Jun. Observation of morphology in Escherichia coli with atom microscope during the cefmetazole-induced post-antibiotic effect period[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(10): 1123-1127.

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