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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2004, Vol. 9 ›› Issue (11): 1236-1238.

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Effects of rAAV/rSTAP on mRNA level of interstitial collagenase in rat stellate cells

LI Xin-Yan1,2, XU Rui-An2, WANG Guang-Ji1, XIE Hai-Tang1, ZHAO Xiao-Chen1, CHEN Miao1   

  1. 1Xin-Zhong New Drug Research and Development Center of China Pharmaceutical University, Nanjing 210009, Jiangsu, China;
    2Gene Therapy Lab of Institute of Molecular Biology in the University of Hong kong, Hongkong, China
  • Received:2004-04-19 Revised:2004-08-09 Online:2004-11-26 Published:2020-11-19

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Abstract: AIM: To observe tlie effects of rAAV/ rSTAP on mRNA level of interstitial collagenase in rat stellate cell. METHODS: Recombinant adeno-associated virus including STAP gene was constructed and obtained. The stellate cells were isolated from SD rat5 s liver and cultured in DMEM medium. The effects of STAP on the level of interstitial collagenase were measured by RT-PCR. RESULTS: The level of interstitial collagenase in rAAV/rSTAP treated cells increased significantly com-pared with that in control group. CONCLUSION: rAAV/rSTAP can up regulate the expressions of interstitial collagenase mRNA and may be associated with anti-hepat-ic fibrosis.

Key words: rAAV/rSTAP, stellate cell activation-associated protein, hepatic stellate cell, interstitial colla-genase

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