Abstract: AIM: To investigate the effects of pene-tration enhancer 1-menthol and azone on percutaneous ab-sorption of bifonazole in vitro. METHODS: Percutane-ous penetration experiment was performed on device in vitro and deposit effect was observed by vivo perception. Orthogonal function spectrophotomery was used for obliter-ating the interferance of absorption RESULTS: Azone (1%) had a significantly effect on percutaneous absorption of bifonazole. The lag times were shortened and the deposit effect was increased by azone. CONCLUSION: Azone can enhance the percutaneous absorption of bifon-azole. Menthol can not enhance the percutaneous absorp-tion of bifonazole, but it can increase the deposit effect.

Key words: 1-menthol, azone, bifonazole, percuta-neous absorption, orthogonal function spectrophotomery