Induction effects of antisense phosphorothioate oligodeoxynucleotides of livin mRNA on apoptosis in MCF-7 cells

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2004, Vol. 9 ›› Issue (12): 1353-1356.

Previous Articles Next Articles

Induction effects of antisense phosphorothioate oligodeoxynucleotides of livin mRNA on apoptosis in MCF-7 cells

ZHANG Hua-Dong, YUAN Shou-Jun, CHEN Hui-Peng, TIAN Zeng-Yue, HAN Chang-Ming

Department of Pharmacology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China

Received:2004-09-07 Revised:2004-10-12 Online:2004-12-26 Published:2020-11-19
Contact: YUAN Shou-Jun, male, professor, specialized in cancer pharmacology.Tel:010^-66930271 E-mail:yuansj@nic.bmi.ac.cn
About author:ZHANG Hua-Dong, male, PhD candidate.

Abstract

Abstract: AIM: To design antisense phosphorothioate oligodeoxynucleotides (PS-ODNs) which targeted to livin mRNA and investigate the anti-cancer effects and the mechanism in vitro.METHODS: Several antisense PS-ODNs were designed and exposed to MCF-7 breast cancer cells.MTT, RT-PCR, flow cytometry and caspase-3 assay were tested in the experiment.RESULTS: The level of livin mRNA was decreased and the activity of caspase3 was increased by YMZ05 in several PS-ODNs designed.The growth of MCF-7 was inhibited and the cell apoptosis was induced by PS-ODN.CONCLUSION: The inhibition of livin can inhibit the growth of MCF-7 breast cancer cells and induce the apoptosis of it.The livin may become a new potential target of anticancer therapy.

Key words: antisense, phosphorothioate oligodeoxynucleotides (PS-ODNs), livin mRNA, MCF-7 cell, cell apoptosis

CLC Number:

R966

ZHANG Hua-Dong, YUAN Shou-Jun, CHEN Hui-Peng, TIAN Zeng-Yue, HAN Chang-Ming. Induction effects of antisense phosphorothioate oligodeoxynucleotides of livin mRNA on apoptosis in MCF-7 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(12): 1353-1356.

[1]	ZHANG Xuejiao, LIU Jieting, LI Luxin, CHEN Peijian, DING Minglu, SUN Mengwei, CHU Yanhui, ZHANG Zhen . Effects and mechanism of dapagliflozin on myocardial injury in type 1 diabetes mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 257-265.
[2]	DONG Wei, TANG Xilan, ZHAO Guowei, JIANG Qieying, LIAO Zhenggen, LIANG Xinli. Effects of coumarins from Angelica dahurica on chemotherapeutic sensitivity of human breast cancer cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(2): 140-146.
[3]	. Effects and mechanisms of nicotinic acid curcumin on apoptosis induced by low shear stress in human umbilical vein endothelial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(8): 855-861.
[4]	MA Chenghua, ZHOU Haijing, SHI Wen, WANG Zhiqiang, YANG Lan, CHEN Ping, ZHENG Xueli, MA Rui, MA Lijuan. Mechanism of apoptosis of mucoepidermoid carcinoma MEC-1 cells induced by ethanol extract of Lamiophlomis rotate [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(3): 253-259.
[5]	LUO Ziyin, XIANG Bingqian, GAO Hui, QIU Xiaoxiao, QIAN Xiaoying, WANG Wantie. Effects of dexmedetomidine on JNK in A549 cells with hypoxia/reoxygenation injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(12): 1346-1351.
[6]	SHAO Mei-qin, CHEN Hai-e, HE Jin-bo, MA Ying-chun, HUANG Lin-jing, CHEN Dan, WANG Yang, WANG Wan-tie. Effects of JNK on ischemic postconditioning attenuating pneumocyte apoptosis after lung ischemia/reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(5): 531-536.
[7]	ZHOU Yan-qiong, ZHANG Yan-mei, GAO Fen-fei, HUANG Zhan-qin, CHEN Yi-cun, ZHENG Yan-shan, SHI Gang-gang. The protective effects of Egr-1 antisense oligodeoxynucleotides on endothelial injury induced by anoxia-reoxygenation [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(1): 14-18.
[8]	ZHOU Jie, YAN Ling, HUANG Duo-xin. Mfn2 gene transfer inhibites neonatal rat cardiomyocytes apoptosis induced by ischemia/reperfusion via activation of the Ras-PI3K-Akt signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 973-978.
[9]	WANG Ming, ZHANG Yao, XIE Xiang-rong, QI Zhi-lin, BI Fu-yong. Oridonin induces MDA-MB-231 cells apoptosis through PI3K/Akt pathway in vitro [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(2): 161-165.
[10]	ZHANG Hai-ying, XU Shui-ling, ZHANG Li-heng. Effects of quercetins extracted and isolated from flos sophorae immaturus and apoptosis in MCF-7 human breast cancer cell in vitro [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(9): 995-1000.
[11]	JIANG Sheng, CHEN Fei-hu, CHEN Cun-zhi, RONG Chao, HU Wei, WU Fan-rong, GE Jing-fang, TANG Jie. Effects of ASIC1a gene silencing by siRNA on apoptosis of articular cartilage cells of adjuvant arthritis rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(3): 256-262.
[12]	SUN Chuan-bo,GAO Jing-hong, HU Liang, ZHANG Jing-wen, WEI Li, LI Qing-ping. High glucose-induced apoptosis of H9c2 cells is closely related to GAPDH nuclear translocation [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(4): 374-379.
[13]	ZHAO Can-guo, HE Yan-jie, ZHOU Sheng-ming. Effects of triptolide on proliferation and apoptosis in MCF-7 human breast cancer cell in vitro [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(2): 135-138.
[14]	TAN Ting, WANG Mei-na, MA Xiao-ya. Effects of Cyproheptadine on cerebral ischemia-reperfusion injury and nerve cell apoptosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(10): 1096-1100.
[15]	ZHU Jing, HONG Hua-rong, LIU Xiao-qun, ZHOU Xiao-li, CHEN Jian-li. Study on the signal pathway of cell apoptosis in HT-29 cells and the gene expression of AQP3 induced by sodium butyrate [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(8): 895-899.

Induction effects of antisense phosphorothioate oligodeoxynucleotides of livin mRNA on apoptosis in MCF-7 cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments