Effects of aspirin and sulindac on expression of cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, and CD44V6 in 3AO cells

Abstract

Abstract: AIM: To investigate the effects of aspirin and sulindac on the expression of cyclooxygenase-2(COX-2), vascular endothelial growth factor (VEGF), CD₄₄V₆ and laminin receptor (LN-R) in 3AO cells. METHODS: The growth inhibition effects of aspirin and sulindac in 3AO cells were tested by MTT assay.The expression of COX-2, VEGF, LN-R and CD₄₄V₆were evaluated by immunocytochemistry.RESULTS: Aspirin and sulindac significantly inhibited the growth of 3AO cells whether added before or after cells adhered.When the pH was adjusted with sodium bicarbonate, the inhibition had a little decrease.Aspirin and sulindac also inhibited the expression of COX-2 and VEGF.But there were no effect on the expression of LN-R and CD₄₄V₆.CONCLUSION: Inhibited expression of COX-2 may down-regulate the expression of VEGF, and inhibit the angiogenesis of tumor.Aspirin and sulindac may exert their chemopreventive and anticancer effects by inhibiting the expressions of COX-2 and VEGF.

Key words: aspirin, sulindac, cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, CD₄₄V₆

CLC Number:

R971.1

GAO Xue-Qin, HAN Jin-Xiang, ZHANG Wei-Dong, SONG Shou-Qin, ZHU Bo. Effects of aspirin and sulindac on expression of cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, and CD₄₄V₆ in 3AO cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(8): 906-910.

References

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Effects of aspirin and sulindac on expression of cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, and CD₄₄V₆ in 3AO cells

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