Abstract: AIM: To investigate the effects of FFBHQH on the level of expression of caspase-3 protein of middle cerebral artery in ischemia reperfusion model and to study its mechanisms in preventing and treating ischemic apoplexy.METHODS: The model of focal cerebral ischemia reperfusion in rats was established with the suture-occluded method.The effects of FFBHQH on the behavior obstacle of rats after MCAO/R 24, 48, and 72 h were observed, and the levels of expression of caspase-3 protein were observed by immunoassay method in rats treated with FFBHQH after the cerebral ischemia reperfusion.RESULTS: Different degrees of behavior obstacle appeared in rats after MCAO/R 24, 48, 72 h, and FFBHQH reduced the behavior obstacle grade.After MCAO/R, the expression of caspase-3 protein increased obviously, but FFBHQH reduced the expression.CONCLUSION: FFBHQH can prevent and treat the ischemic apoplexy, and the mechanisms may relate to the calcium antagonism and the prevention of the apoptosis correlated gene such as caspase-3 after cerebral ischemia.

Key words: cerebral infarction, ischemia reperfusion, FFBHQH, rats, caspase-3