Abstract: AIM: To explore the effects of ASODN targeting survivin on apoptosis in HL-60 cells.METHODS: The cell proliferation was tested by MTT assay, the cell morphological transformation was observed by inverted microscope, the cell apoptosis index was examined by TUNEL, the cell apoptosis rate was precisely detected by flow cytometry, and the expression of survivin mRNA was detected by reverse transcriptase PCR.RESULTS: 5-20 μmol·L^-1of survivin ASODN showed obviously inhibitory effect on the cell proliferation of HL-60 cells, and the inhibitory effect correlated with time and dosage.The cell apoptosis rate and the expression inhibitory rate of survivin gene in ASODN groups were obviously higher than that in the control group.CONCLUSION: ASODN targeting survivin can effectively inhibit the expression of survivinmRNA and induce the cell apoptosis, and it indicates that survivin plays an important role in maintaining the proliferation of tumor cells.

Key words: antisense oligonucleotide, HL-60 cell, apoptosis, inhibitor of apoptosis protein