Abstract: AIM: To explore the effect and mechanism on blood pressure and heart protection of renal hypertension in rats treated with β₁ receptor antisense oli-godeoxynucleotides (β₁-AS-ODN) by delivery with cation-icliposomes DOTAP/DOPE.METHODS: 24 SD rats were divided randomly into 4 groups of 6 each group two groups of 2KIC rats were treated via tail vein injection with β₁-AS-ODN or inverted oli gonucleotides against rat β₁-AR MNA (β₁-IN-ODN) of 0.5 mg·kg^-1 once. 6 unltreated 2K1C rats and shamed rats served as positive andnormal controls. Blood pressure (BP), cardiac haemodynamics, and left ventricular index (LVW/BW) were measured. The histological changes of myocardium were observed by optical microscope and the expressions of Bcl-2 and Bax in myocardium were examined by immunohistochemical method.RESULTS: The single dose management of β₁-AS-ODN decreased blood pressure to 120 mm-Hg for 27 days and improved the LV function obviously P < 0.01). The value of LVW/BW in β₁-AS-ODN oup is much less than the value of that in 2K1C group(P 0.01). Compared with that of 2K1C group, thethological changes of myocardium inβ₁-AS-ODN groupmeliorated. The radio of bcl-2/Bax in 2KIC group wasless than sham group, and the radio of that in β₁-AS-ODN group was more than 2KIC group (P < 0. 05).CONCLUSION: β₁ receptor antisense oligodeoxynucleotidescan maintain anti-hypertension effect for atime and improve LV function, effectively reverses left ventricular hypertrophy in 2K1C renal hypertensive rats. The mecha-nism of which may be related to the suppressing of cell apoptosis of myocardium by increasing Bcl-2/Bax. It sug-gests that, receptor mRNA antisense had the heart protection effect in renal hypertensive raf.

Key words: renal hypertension, antisense oligonucleotides, β₁-adrenergic receptor, gene therapy, left ventricular hypertrophy, heart protection, apotosis, Bcl-2/Bax