Abstract: AIM: To establish a new sensitive micro-late-based method to determine alpha-glucosidase inhibiting activity and provide a reliable high-through put way for screening alpha-glucosidase inhibitors in vitro.METHODS: The fitting combination of enzyme and sub-strate in a certain reaction was tested. Acarbose, the most popular alpha-glucosidase inhibitor in clinical use was used to validate the established method. Calibration curve, wavelength fidelity and kinetic analysis, together with the effect of altered incubation time, temperature and PH were then studied.RESULTS: The details of assayprocedure and evaluation of factors affecting thee measure-ment are described. As little as 160 μl assaythe combination of 2.5 mmol·L^-1 PNPGU and alpha-glucosidase was performed in a 96-well plate. The optimal action was finally achieved by incubat-ed at 37 C, PH 7.0 for 15 min and measured at 400nm.RESULTS: from the validation exercises by Acartosestrongly demonstrated the accuracy and reliability of the pmposed approach.CONCLUSION: This method re-ported in the current paper makes it possible to rapidlyexamine large numbers of samples for the presence of al-pha-glucosidase inhibitors in very small sample volume Such action may help to pace the development of potentialoral medications from natural products protecting patienagainst postprandial hyperglylyceruic toxicity andoretreating (iabetes mellitus and the related complications.

Key words: acarbose, alpha-glucosidase inhibitorsdrug screening, microplate assay: postprandial hyperglycemla