Abstract: AIM: To establish sensitive DBT cell model to MHV-A59 by virology method,and develoPse-lective method of anti virus drugs in cell level.METHODS: Test 50% percent tissue culture infective dose(TCID₅₀)by cytopathetic effect(CPE)and plaque meth-ods.Preparation of antibody against virus by partially pu-rified virus immunized animal.Selective anti virus drug adding 100 fold of TICD50virus dose infected DBT cell as positive results by protesting lower50%CPE,and tested drugs lowest virus inhibit concentration.RESULTS: Pre-pared antibody has specificity reverse virus action,and new synthesized drugs had various degree protective ac-tions to virus infective.CONCLUSION: The DBT cell model sensitive to MHV-A59 can select anti coronaviruses drug.

Key words: MHV-A59, DBT cell, TCID50, ELISA, antibody