Abstract: AIM: To investigate the inhibition ability of recombinant Oncostatin M(OSM) to proliferation and migration of tumorcells.METHODS: Expressed and purified human OSM from recombinant E.Coli,and as-sayed its pharmacology activity by a series of experiments in vitro to metastatic human lung cancercell line 95-D including MTT assay,clone formationassay in soft agar,cell attachment assay,cell migration assay and invasion cuPassay.RESULTS AND CONCLUSION: rhOSM displayed strong activity of inhibiting the proliferation and migration of 95-D.Prokaryoticly expressed hOSM has po-tential clinical value to lung cancer.

Key words: oncostatin M, metastatic human lung cancercell line 95-D, metastasis