Abstract: AIM: To evaluate the protection of Mn²⁺ on injury of human kidney tubularepithelial cell line in-duced by gentamicin,and investigate its principals.METHODS: Gentamicin was used to injury human kid-ney tubularepithelial cell line (HK-2) to produce model of kidney injury.MTT method was used to measure effect of Mn2+ on proliferation of cells afterthey were injured by gentamicin.The spectrophotometry method was used to observe the change of LDH,NAG and SOD effected by Mn²⁺.Ultrastructure was examined by electron microsco-py.RESULTS: Although HK-2 cells were injured,Mn²⁺ promoted cells proliferation,decreased LDH activity and NAG content,increased SOD activity,and alleviated dilation of the mitochondria and crushing of lysosomes.CONCLUSION: Mn²⁺ can protect human kidney tubularepithelial cell line from injury induced by gentamicin.It may be in relation to inhibiting the oxidative injury,light-ening dilation of the mitochondria,protecting the integrity of lysosomes and decrease the leakage of enzyme.

Key words: Mn²⁺, gentamicin, kidney tubalarepi-thelid cell, oxidative injury, oxygen free radicals