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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2005, Vol. 10 ›› Issue (12): 1372-1375.

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Argument foreffect of Vitamin K3 applying to fungal counting with MTT assay

FANG Shi-jie, ZHU Jing, WANG Xu   

  1. Life Science Faculty of Yunnan Normal University,Kunming 650092,Yunnan,China
  • Received:2005-09-12 Revised:2005-10-12 Online:2005-12-26 Published:2020-11-11

Abstract: AIM: To determine the concentration of vitamin K3 (VK3) in fungal counting with MTT assay.METHODS: Microplate andMTT colorimetric assay were used to measure the effect of different VK3 concentrations on fungal counting.RESULTS: The OD(optical density)value was affected by fungal and VK3 concentration,measure wavelengh,and solvent addition-time at the same time.Generally speaking,the OD value was increased with fungal and VK3 concentration increased.At a higherfungal concentration,there was little impact from solvent addition-time and remarkably impact from measure wavelength.CONCLUSION: The reliable and stable results will be acquired in the conditions as follows: 106 fungal concentration,0.1 mol·L-1 VK3 concentration,and 570 nm measure wavelengh.

Key words: VK3, MTT assay, fungi, fungal counting

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